a Department of Property Protection and Safety , Osmancık Ömer Derindere Vocational Higher School, Hitit University , Çorum , Turkey.
Artif Cells Nanomed Biotechnol. 2017 Feb;45(1):31-38. doi: 10.1080/21691401.2016.1215326. Epub 2016 Aug 10.
The separation and purification methods are extremely important for the hemoglobin (Hb) which is a crucial biomolecule. The adsorption technique is popular among these methods and the cryogels have been used quite much due to their macropores and interconnected flow channels. In this study, the Hb adsorption onto the Cu(II) immobilized poly(2-hydroxyethyl methacrylate-glycidyl methacrylate), poly(HEMA-GMA)-Cu(II), cryogels was investigated under different conditions (pH, interaction time, initial Hb concentration, temperature and ionic strength) to optimize adsorption conditions. The swelling test, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscope (SEM), surface area (BET), elemental and ICP-OES analysis were performed for the characterization of cryogels. Polyethyleneimine (PEI) molecule was used as a Cu(II)-chelating ligand. The Hb adsorption capacity of cryogels was determined as 193.8 mg Hb/g cryogel. The isolation of Hb from human blood was also studied under optimum adsorption conditions determined and the Hb (124.5 mg/g cryogel) was isolated. The adsorption model was investigated in the light of Langmuir and Freundlich adsorption isotherm models and it was determined to be more appropriate to the Langmuir adsorption isotherm model.
血红蛋白（Hb）是一种重要的生物分子，其分离和纯化方法极其重要。在这些方法中，吸附技术很流行，由于具有大孔和相互连通的流道，因此使用了很多冷冻凝胶。在这项研究中，研究了在不同条件（pH、相互作用时间、初始 Hb 浓度、温度和离子强度）下将 Cu(II)固定在聚（2-羟乙基甲基丙烯酸酯-甲基丙烯酸缩水甘油酯）、聚（HEMA-GMA）-Cu(II)、冷冻凝胶上的 Hb 吸附情况，以优化吸附条件。进行了溶胀试验、傅里叶变换红外（FT-IR）光谱、扫描电子显微镜（SEM）、表面积（BET）、元素和 ICP-OES 分析，以对冷冻凝胶进行表征。聚乙烯亚胺（PEI）分子用作 Cu(II)螯合配体。冷冻凝胶的 Hb 吸附容量确定为 193.8 mg Hb/g 冷冻凝胶。还在确定的最佳吸附条件下研究了从人血中分离 Hb，分离出的 Hb（124.5 mg/g 冷冻凝胶）。根据 Langmuir 和 Freundlich 吸附等温线模型研究了吸附模型，结果表明该模型更适合 Langmuir 吸附等温线模型。
