Kim Rae Young, Chung Sung Kun, Kim Man Soo, Ra Ho
Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Cornea. 2016 Dec;35(12):1615-1620. doi: 10.1097/ICO.0000000000001016.
To evaluate the effects of combined photodynamic therapy (PDT) and topical bevacizumab treatment on corneal neovascularization in rabbits.
Corneal neovascularization was induced by placing a suture for 7 days in both eyes of 15 rabbits. After suture removal, the right eyes of the rabbits were divided into 3 treatment groups (n = 5 each): group 1, 5 mg/mL bevacizumab eye drops; group 2, PDT at 150 J/cm; and group 3, combined PDT and topical bevacizumab treatment. All 15 left eyes formed a control group. After 2 weeks of treatment, digital photographs were obtained and analyzed to determine the area of corneal neovascularization. The rabbits were then killed to measure vascular endothelial growth factor (VEGF) levels in corneal tissues.
The corneal neovascularization area after treatment was significantly smaller in the 3 treatment groups compared with that in the control group. A significant difference was observed between groups 1 and 2 and group 3, but not between groups 1 and 2. VEGF levels were significantly lower in groups 1 and 3 than in the control group. Although group 2 exhibited relatively higher VEGF levels compared with the control group, the difference was not significant.
Our results suggest that combined PDT and topical bevacizumab treatment is significantly more effective than monotherapy for the treatment of corneal neovascularization in rabbits. Such quantitative comparison in the laboratory is believed to provide the theoretical basis for clinical application of combined PDT and topical bevacizumab treatment.
评估光动力疗法（PDT）联合局部应用贝伐单抗治疗对兔角膜新生血管化的影响。
对15只兔双眼放置缝线7天诱导角膜新生血管化。拆线后，将兔右眼分为3个治疗组（每组n = 5）：第1组，5 mg/mL贝伐单抗滴眼液；第2组，150 J/cm的PDT；第3组，PDT联合局部应用贝伐单抗治疗。所有15只左眼形成对照组。治疗2周后，获取数码照片并分析以确定角膜新生血管化面积。然后处死兔以测量角膜组织中血管内皮生长因子（VEGF）水平。
与对照组相比，3个治疗组治疗后的角膜新生血管化面积明显更小。第1组与第2组和第3组之间观察到显著差异，但第1组和第2组之间无显著差异。第1组和第3组的VEGF水平明显低于对照组。虽然第2组与对照组相比VEGF水平相对较高，但差异不显著。
我们的结果表明，PDT联合局部应用贝伐单抗治疗在治疗兔角膜新生血管化方面明显比单一疗法更有效。实验室中的这种定量比较被认为可为PDT联合局部应用贝伐单抗治疗的临床应用提供理论依据。
