Demir Selim, Turan Ibrahim, Aliyazicioglu Yuksel
Department of Nutrition and Dietetics, Faculty of Health Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
J BUON. 2016 Jul-Aug;21(4):883-888.
The genus Rhododendron is distributed entirely in the world with the exception of South and Central America and Africa, growing in a large diversity of climatic conditions. This genus is a rich source of phenolic compounds, especially flavonoids, essential oils, chromones, terpenoids, and steroids. It has many biological properties such as antioxidant, anti-inflammatory, antiviral, antibacterial, anticancer, antidiabetic, immunomodulatory, cardioprotective and hepatoprotective among others due to their polyphenolic constituents. The objective of the current study was to evaluate the antioxidant properties and cytotoxic activity of dimethyl sulfoxide extract of flowers of Rhododendron luteum (DEFR) for the first time.
The total polyphenolic contents (TPC), total flavonoid contents (TFC) and ferric reducing antioxidant power (FRAP) of the extract were evaluated using spectrophotometric procedures. The cytotoxic activity of the extract on three cancers (human breast, colon and liver carcinoma) and human foreskin fibroblast cells was determined using the MTT assay.
TPC and FRAP values were found 54.2±0.38 mg gallic acid equivalents and 164.2±1.77 mg trolox equivalents per to g sample, respectively. R.luteum extract exhibited selective cytotoxicity against colon and liver cancer cells compared to normal fibroblast cells, while this selective cytotoxicity was not observed in breast cancer cells.
Our results demonstrate that the Rhododendron luteum may be a great source of antioxidant and antitumor natural agents due to their capability of decreasing cancer cells proliferation.
杜鹃花属植物除在南美洲、中美洲和非洲外，在全球均有分布，生长于多种气候条件下。该属植物富含酚类化合物，尤其是黄酮类化合物、精油、色酮、萜类化合物和甾体化合物。由于其多酚成分，它具有许多生物学特性，如抗氧化、抗炎、抗病毒、抗菌、抗癌、抗糖尿病、免疫调节、心脏保护和肝脏保护等。本研究的目的是首次评估淡黄杜鹃花朵的二甲基亚砜提取物（DEFR）的抗氧化性能和细胞毒性活性。
采用分光光度法评估提取物的总多酚含量（TPC）、总黄酮含量（TFC）和铁还原抗氧化能力（FRAP）。使用MTT法测定提取物对三种癌症（人乳腺癌、结肠癌和肝癌）和人包皮成纤维细胞的细胞毒性活性。
TPC值和FRAP值分别为每克样品54.2±0.38毫克没食子酸当量和164.2±1.77毫克 Trolox当量。与正常成纤维细胞相比，淡黄杜鹃提取物对结肠癌细胞和肝癌细胞表现出选择性细胞毒性，而在乳腺癌细胞中未观察到这种选择性细胞毒性。
我们的结果表明，由于淡黄杜鹃具有降低癌细胞增殖的能力，它可能是抗氧化和抗肿瘤天然药物的重要来源。
