Liu Yuxiang, Hu Tingting, Zhao Jing, Lv Yongkang, Ren Ruipeng
College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
Key Laboratory of Coal Science and Technology of Shanxi Province and Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China.
J Biosci Bioeng. 2017 Feb;123(2):223-229. doi: 10.1016/j.jbiosc.2016.08.009. Epub 2016 Sep 27.
A novel heterotrophic nitrifying fungus, defined as Penicillium sp. L1, can form mycelial pellets in liquid medium in this study. The effects of inoculation method, C/N ratio, initial pH, and temperature were gradually evaluated to improve the simultaneous removal of total nitrogen (TN) and chemical oxygen demand (COD) in wastewater by Penicillium sp. L1. Results showed that compared with spore inoculation, 48 h pellet inoculum could significantly increase the pellet size (from about 1.5 mm to 3.2 mm) and improve the removal capability, particularly for COD removal (from less than 50-86.20%). The removal efficiencies of TN and COD reached 98.38% (from 136.01 mg/L to 2.20 mg/L) and 92.40% (from 10,720 mg/L to 815 mg/L) under the following conditions: C/N 36, pH 3, 30°C, and inoculation with 48 h pellets. The pellet diameter reached 4.8 mm after 4-day cultivation. In this case, Penicillium sp. L1 removed TN from 415.93 mg/L to 43.39 mg/L, as well as COD from 29,533 mg/L to 8850 mg/L. Overall, the results indicated that the pellet size was closely related to the pollutant-removal ability of Penicillium sp. L1. Furthermore, mycelial pellets (4.8 mm, dead) only adsorbed 38.08% TN (from 125.45 mg/L to 77.78 mg/L), which indicated that adsorption did not play a major role in the nitrogen-removal process.
在本研究中，一种新的异养硝化真菌，被定义为青霉菌L1，能够在液体培养基中形成菌丝球。逐步评估了接种方法、碳氮比、初始pH值和温度对青霉菌L1同步去除废水中总氮（TN）和化学需氧量（COD）的影响。结果表明，与孢子接种相比，48小时的菌球接种可显著增加菌球尺寸（从约1.5毫米增加到3.2毫米）并提高去除能力，特别是对COD的去除（从低于50%提高到86.20%）。在以下条件下：碳氮比36、pH值3、30°C以及接种48小时的菌球，TN和COD的去除效率分别达到98.38%（从136.01毫克/升降至2.20毫克/升）和92.40%（从10,720毫克/升降至815毫克/升）。培养4天后菌球直径达到4.8毫米。在这种情况下，青霉菌L1将TN从415.93毫克/升降至43.39毫克/升，同时将COD从29,533毫克/升降至8850毫克/升。总体而言，结果表明菌球尺寸与青霉菌L1的污染物去除能力密切相关。此外，菌丝球（4.8毫米，死亡）仅吸附了38.08%的TN（从125.45毫克/升降至77.78毫克/升），这表明吸附在脱氮过程中不起主要作用。
