Research Area Biochemical Engineering, Institute of Chemical Engineering, TU Wien, Gumpendorfer Straße 1a, 1060, Vienna, Austria.
Christian Doppler Laboratory for Mechanistic and Physiological Methods for Improved Bioprocesses, TU Wien, Gumpendorfer Straße 1a, 1060, Vienna, Austria.
Appl Microbiol Biotechnol. 2018 Apr;102(7):2997-3006. doi: 10.1007/s00253-018-8818-7. Epub 2018 Feb 22.
Filamentous fungi are used for the production of a multitude of highly relevant biotechnological products like citric acid and penicillin. In submerged culture, fungi can either grow in dispersed form or as spherical pellets consisting of aggregated hyphal structures. Pellet morphology, process control and productivity are highly interlinked. On the one hand, process control in a bioreactor usually demands for compact and small pellets due to rheological issues. On the other hand, optimal productivity might be associated with less dense and larger morphology. Over the years, several publications have dealt with aforementioned relations within the confines of specific organisms and products. However, contributions which evaluate such interlinkages across several fungal species are scarce. For this purpose, we are looking into methods to manipulate fungal pellet morphology in relation to individual species and products. This review attempts to address (i) how variability of pellet morphology can be assessed and (ii) how morphology is linked to productivity. Firstly, the mechanism of pellet formation is outlined. Subsequently, the description and analysis of morphological variations are discussed to finally establish interlinkages between productivity, performance and morphology across different fungal species.
丝状真菌被用于生产多种具有重要意义的生物技术产品，如柠檬酸和青霉素。在液体深层培养中，真菌可以以分散的形式生长，也可以形成由聚集的菌丝结构组成的球形颗粒。颗粒形态、过程控制和生产力高度相关。一方面，由于流变学问题，生物反应器中的过程控制通常需要紧凑和小的颗粒。另一方面，最佳生产力可能与密度较低和较大的形态相关。多年来，已有多篇文献在特定生物体和产品的范围内处理了上述关系。然而，评估跨几种真菌物种的这些相互关系的贡献却很少。为此，我们正在研究与个别物种和产品相关的操纵真菌颗粒形态的方法。本文综述了（i）如何评估颗粒形态的可变性，以及（ii）形态如何与生产力相关。首先，概述了颗粒形成的机制。随后，讨论了形态变化的描述和分析，最终建立了不同真菌物种之间生产力、性能和形态之间的相互关系。
