Institute for Clean Energy & Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing 400715, PR China.
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Biosens Bioelectron. 2017 Jan 15;87:998-1004. doi: 10.1016/j.bios.2016.09.066. Epub 2016 Sep 20.
Monitoring superoxide anion radicals in living cells has been attracting much academic and industrial interest due to the dual roles of the radicals. Herein, we synthesized a novel nanostructured cobalt phosphate nanorods (Co(PO) NRs) with tunable pore structure using a simple and effective micro-emulsion method and explored their potential utilization in the electrochemical sensing of superoxide anions. As an analytical and sensing platform, the nanoscale biomimetic enzymes Co(PO) NRs exhibited excellent selectivity and sensitivity towards superoxide anion (O) with a low detection limit (2.25nM), wide linear range (5.76-5396nM), and long-term stability. Further, the nanoscale biomimetic enzyme could be efficiently applied in situ to electrochemically detect O released from human malignant melanoma cells and normal keratinocyte, showing excellent real time quantitative detection capability. This material open up exciting opportunities for implementing biomimetic enzymes in nanoscale transition metal phosphates and designing enzyme-free biosensors with much higher sensitivity and durability in health and disease analysis than those of natural one.
由于自由基的双重作用，监测活细胞中的超氧阴离子自由基一直引起学术界和工业界的极大兴趣。本文采用简单有效的微乳液法合成了一种具有可调孔结构的新型纳米结构磷酸钴纳米棒（Co(PO) NRs），并探索了其在超氧阴离子电化学传感中的潜在应用。作为分析和传感平台，纳米仿生酶 Co(PO) NRs 对超氧阴离子（O）具有优异的选择性和灵敏度，检测限低（2.25nM），线性范围宽（5.76-5396nM），稳定性好。此外，纳米仿生酶可有效原位应用于电化学检测人恶性黑素瘤细胞和正常角质形成细胞释放的 O，具有优异的实时定量检测能力。该材料为在纳米尺度过渡金属磷酸盐中实施仿生酶以及设计比天然酶具有更高灵敏度和耐久性的无酶生物传感器在健康和疾病分析中开辟了令人兴奋的机会。
