Mazaki-Tovi M, Bolin S R, Schenck P A
Department of Pathobiology and Diagnostic Investigation, Diagnostic Center for Population and Animal Health College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Department of Pathobiology and Diagnostic Investigation, Diagnostic Center for Population and Animal Health College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA.
Vet J. 2016 Oct;216:136-41. doi: 10.1016/j.tvjl.2016.08.002. Epub 2016 Aug 10.
This study aimed to determine the effects of body condition, fat depot, and a peroxisome proliferator-activated receptor γ-agonist (troglitazone) on secretion of adiponectin, interleukin-6 (IL6), and tumor necrosis factor-α (TNFα) from adipose tissue of healthy dogs. Subcutaneous and omental visceral adipose tissue samples were collected from 16 healthy intact female dogs, and body condition score (range 4-8/9) was determined. Concentrations of adiponectin were measured in mature adipocytes cultures and concentrations of IL6 and TNFα were measured in stromovascular cells cultures after 48 h incubation in fresh control medium, or fresh medium containing 10 µM troglitazone. Mature adipocytes and stromovascular cells of subcutaneous origin secreted higher concentrations of adiponectin and lower concentration of IL6 and TNFα, respectively, than corresponding cells of visceral origin, in both the control (P = 0.015, P = 0.004, and P = 0.016, respectively) and troglitazone-treated cultures (P <0.001, P = 0.004, and P = 0.016, respectively). Troglitazone increased adiponectin secretion from mature adipocytes in visceral (P = 0.019), but not in subcutaneous fat cultures (P = 0.4). Troglitazone decreased IL6 and TNFα secretion from stromovascular cells both in visceral (P = 0.047 and P = 0.016, respectively) and subcutaneous (P = 0.047 and P = 0.016, respectively) fat cultures. Higher body condition score was associated with lower secretion of adiponectin from mature adipocytes (P = 0.007), lower secretion of IL6 (P = 0.040) and higher secretion of TNFα (P = 0.040) from stromovascular cells. This study showed differential secretion of adipokines by subcutaneous and visceral fat depots in dogs and association between body condition and adipokine secretion. Activation of PPARγ altered adipokine secretion.
本研究旨在确定身体状况、脂肪储存部位以及过氧化物酶体增殖物激活受体γ激动剂（曲格列酮）对健康犬脂肪组织中脂联素、白细胞介素-6（IL6）和肿瘤坏死因子-α（TNFα）分泌的影响。从16只健康未绝育的雌性犬采集皮下和网膜内脏脂肪组织样本，并确定身体状况评分（范围为4 - 8/9）。在新鲜对照培养基或含有10µM曲格列酮的新鲜培养基中孵育48小时后，测量成熟脂肪细胞培养物中脂联素的浓度以及基质血管细胞培养物中IL6和TNFα的浓度。在对照培养物（分别为P = 0.015、P = 0.004和P = 0.016）和曲格列酮处理的培养物（分别为P <0.001、P = 0.004和P = 0.016）中，皮下来源的成熟脂肪细胞和基质血管细胞分别比内脏来源的相应细胞分泌更高浓度的脂联素和更低浓度的IL6及TNFα。曲格列酮增加了内脏成熟脂肪细胞中脂联素的分泌（P = 0.019），但在皮下脂肪培养物中未增加（P = 0.4）。曲格列酮降低了内脏（分别为P = 0.047和P = 0.016）和皮下（分别为P = 0.047和P = 0.016）脂肪培养物中基质血管细胞分泌的IL6和TNFα。较高的身体状况评分与成熟脂肪细胞中脂联素分泌降低（P = 0.007）、基质血管细胞中IL6分泌降低（P = 0.040）以及TNFα分泌增加（P = 0.040）相关。本研究表明犬皮下和内脏脂肪储存部位脂联素分泌存在差异，且身体状况与脂联素分泌之间存在关联。PPARγ的激活改变了脂联素的分泌。
