Mazaki-Tovi Michal, Bolin Steven R, Schenck Patricia A
Department of Pathobiology and Diagnostic Investigation, Diagnostic Center for Population and Animal Health College of Veterinary Medicine, Michigan State University, East Lansing, MI, 48824, USA.
Lipids. 2018 Feb;53(2):205-216. doi: 10.1002/lipd.12021. Epub 2018 Mar 24.
The aim of this study was to determine the effect of n3 polyunsaturated fatty acids (PUFA) on canine adipose tissue secretion of adiponectin, interleukin-6 (IL6), and tumor necrosis factor-α (TNFα). Subcutaneous and omental visceral adipose tissue samples were collected from 16 healthy intact female dogs. Concentrations of adiponectin were measured in mature adipocyte cultures, and concentrations of IL6 and TNFα were measured in undifferentiated stromovascular cell (SVC) cultures following treatment with eicosapentaenic acid (EPA, 20:5n-3), arachidonic acid (ARA, 20:4n-6), or palmitic acid (PAM, 16:0) at 25, 50, or 100 μM. Secretion of adiponectin from mature adipocytes was higher (p < 0.001) following EPA treatment at 50 μM compared to control in subcutaneous tissue, and higher following EPA treatment compared to PAM treatment at 25 μM in both subcutaneous (p < 0.001) and visceral tissues (p = 0.010). Secretion of IL6 from SVC derived from subcutaneous tissue was lower following EPA treatment and higher following PAM treatment compared to control both at 50 μM (p = 0.001 and p = 0.041, respectively) and 100 μM (p = 0.013 and p < 0.001, respectively). These findings of stimulation of adiponectin secretion and inhibition of IL6 secretion by EPA, and stimulation of IL6 secretion by PAM, are consistent with findings of increased circulating concentrations of adiponectin and decreased circulating concentration of IL6 in dogs supplemented with dietary fish oil, and show that the effect of fish oil on circulating concentrations of adiponectin and IL6 is, at least partially, the result of local effects of EPA and PAM on adipose tissue.
本研究的目的是确定n3多不饱和脂肪酸（PUFA）对犬脂肪组织脂联素、白细胞介素-6（IL6）和肿瘤坏死因子-α（TNFα）分泌的影响。从16只健康未绝育的雌性犬采集皮下和网膜内脏脂肪组织样本。在成熟脂肪细胞培养物中测量脂联素浓度，在用25、50或100μM的二十碳五烯酸（EPA，20:5n-3）、花生四烯酸（ARA，20:4n-6）或棕榈酸（PAM，16:0）处理后，在未分化的基质血管细胞（SVC）培养物中测量IL6和TNFα浓度。与皮下组织中的对照相比，50μM EPA处理后成熟脂肪细胞的脂联素分泌更高（p < 0.001），并且在皮下组织（p < 0.001）和内脏组织（p = 0.010）中，与25μM PAM处理相比，EPA处理后的脂联素分泌更高。与对照相比，皮下组织来源的SVC中IL6的分泌在50μM（分别为p = 0.001和p = 0.041）和100μM（分别为p = 0.013和p < 0.001）时，EPA处理后较低，PAM处理后较高。这些关于EPA刺激脂联素分泌和抑制IL6分泌以及PAM刺激IL6分泌的发现，与补充膳食鱼油的犬中脂联素循环浓度升高和IL6循环浓度降低的发现一致，并且表明鱼油对脂联素和IL6循环浓度的影响至少部分是EPA和PAM对脂肪组织局部作用的结果。
