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小鼠Ss蛋白的研究：证明Ss蛋白在功能上是补体的第四成分。

Studies on the murine Ss protein: demonstration that the Ss protein is functionally the fourth component of complement.

作者信息

Carroll M C, Capra J D

出版信息

Proc Natl Acad Sci U S A. 1978 May;75(5):2424-8. doi: 10.1073/pnas.75.5.2424.

DOI:10.1073/pnas.75.5.2424
PMID:276882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392566/
Abstract

This report describes a simple and reproducible assay for the fourth component of murine complement (C4). Utilizing this assay, experiments are described which conclusively demonstrate that the murine Ss protein (serum substance) is functional C4 and that ascitic fluid may be used as a source for isolating Ss. The evidence that Ss is functional C4 is: (i) pretreatment of mouse plasma with F(ab')2 anti-Ss completely abrogated the C4 hemolytic titer; and (ii) in the isolation of the Ss protein from ascitic fluid, Ss immunochemical activity and C4 functional activity coeluted in each step of a four-step purification procedure.

摘要

本报告描述了一种用于检测小鼠补体第四成分（C4）的简单且可重复的检测方法。利用该检测方法，进行了一系列实验，这些实验确凿地证明了小鼠Ss蛋白（血清物质）就是功能性C4，并且腹水可作为分离Ss的来源。证明Ss是功能性C4的证据如下：（i）用F(ab')2抗Ss预处理小鼠血浆可完全消除C4溶血效价；（ii）在从腹水中分离Ss蛋白的过程中，在四步纯化程序的每一步中，Ss免疫化学活性和C4功能活性都共同洗脱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e83c/392566/4bd78d11ade8/pnas00017-0379-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e83c/392566/b2b22832147a/pnas00017-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e83c/392566/4bd78d11ade8/pnas00017-0379-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e83c/392566/b2b22832147a/pnas00017-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e83c/392566/4bd78d11ade8/pnas00017-0379-b.jpg

相似文献

1
Studies on the murine Ss protein: demonstration that the Ss protein is functionally the fourth component of complement.小鼠Ss蛋白的研究：证明Ss蛋白在功能上是补体的第四成分。
Proc Natl Acad Sci U S A. 1978 May;75(5):2424-8. doi: 10.1073/pnas.75.5.2424.
2
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J Exp Med. 1977 Oct 1;146(4):1001-8. doi: 10.1084/jem.146.4.1001.
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Variance in the activity of the fourth component of mouse complement.小鼠补体第四成分活性的差异
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Immunochemical characterization of murine H-2 controlled Ss (serum substance) protein through identification of its human homologue as the fourth component of complement.通过鉴定小鼠H-2控制的Ss（血清物质）蛋白的人类同源物为补体第四成分对其进行免疫化学特性分析。
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Evidence for the involvement of the Ss protein of the mouse in the hemolytic complement system.小鼠Ss蛋白参与溶血补体系统的证据。
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Mouse C4 (Ss): three-step purification of its C4c fragment and production of a monospecific antiserum.小鼠C4（Ss）：其C4c片段的三步纯化及单特异性抗血清的制备。
J Immunol. 1979 Feb;122(2):490-3.

引用本文的文献

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Genetic and structural characterization of H-2-controlled allelic forms of murine C4.小鼠C4的H-2控制等位基因形式的遗传与结构特征
Immunogenetics. 1980 Jul;11(1):55-63. doi: 10.1007/BF01567769.
2
A murine C4 molecule with reduced hemolytic efficiency.一种溶血效率降低的小鼠C4分子。
J Exp Med. 1980 Feb 1;151(2):492-7. doi: 10.1084/jem.151.2.492.
3
Partial amino acid sequences of the murine fourth component of complement (C4): demonstration of homology with human C4 and identification of the amino-terminal subunit in pro-C4.

本文引用的文献

1
A Serologically Detected Variant in Mouse Serum: Inheritance and Association with the Histocompatibility-2 Locus.小鼠血清中一种血清学检测到的变体：遗传方式及其与组织相容性-2位点的关联
Genetics. 1963 Jan;48(1):9-25. doi: 10.1093/genetics/48.1.9.
2
The Action of Ammonia on Complement. The Fourth Component.氨对补体的作用。第四成分。
Biochem J. 1926;20(5):1028-35. doi: 10.1042/bj0201028.
3
Diffusion-in-gel methods for immunological analysis.用于免疫分析的凝胶扩散法。
小鼠补体第四成分（C4）的部分氨基酸序列：与人类C4的同源性证明及前体C4中氨基末端亚基的鉴定
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4275-8. doi: 10.1073/pnas.77.7.4275.
4
H-2 S region determined polymorphic variants of the C4, Slp, C2, and B complement proteins: a compilation.H-2 S区域决定的C4、Slp、C2和B补体蛋白的多态性变体：汇编
Immunogenetics. 1982;16(6):617-23. doi: 10.1007/BF00372032.
5
Mouse Slp: female expression due to a dominant non-H-2 gene in wild mice.小鼠Slp：野生小鼠中由显性非H-2基因导致的雌性表达。
Immunogenetics. 1982;15(6):601-4. doi: 10.1007/BF00347054.
6
Murine antigen H-2.7: localization of its antigenic determinant to the Ss (C4) molecule.小鼠抗原H-2.7：其抗原决定簇在Ss（C4）分子上的定位
Immunogenetics. 1980;11(6):605-15. doi: 10.1007/BF01567829.
7
Structural and functional differences between the H-2 controlled Ss and Slp proteins.H-2 控制的 Ss 蛋白和 Slp 蛋白之间的结构与功能差异。
J Exp Med. 1978 Nov 1;148(5):1186-97. doi: 10.1084/jem.148.5.1186.
8
Studies on murine Ss protein: demonstration that S region encodes structural gene for fourth component of complement.小鼠Ss蛋白的研究：证明S区域编码补体第四成分的结构基因。
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4641-5. doi: 10.1073/pnas.76.9.4641.
9
Structural characterization of the murine fourth component of complement and sex-limited protein and their precursors: evidence for two loci in the S region of the H-2 complex.小鼠补体第四成分和性限制蛋白及其前体的结构特征：H-2复合体S区域中两个基因座的证据。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5853-7. doi: 10.1073/pnas.76.11.5853.
Prog Allergy. 1958;5:1-78.
4
Anaphylatoxin release from the third component of human complement by hydroxylamine.羟胺诱导人补体第三成分释放过敏毒素
Science. 1969 Aug 1;165(3892):506-7. doi: 10.1126/science.165.3892.506.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Genetic control of haemolytic complement in mice: association with H-2.小鼠溶血性补体的遗传控制：与H-2的关联。
Folia Biol (Praha). 1972;18(4):237-43.
7
A sex-limited serum protein variant in the mouse: inheritance and association with the H-2 region.小鼠中的一种性别限制血清蛋白变体：遗传及与H-2区域的关联。
Biochem Genet. 1970 Jun;4(3):351-65. doi: 10.1007/BF00485752.
8
Immunochemical quantitation of antigens by single radial immunodiffusion.通过单向辐射免疫扩散法对抗原进行免疫化学定量。
Immunochemistry. 1965 Sep;2(3):235-54. doi: 10.1016/0019-2791(65)90004-2.
9
The role of the histocompatibility-2-linked Ss-Slp region in the control of mouse complement.组织相容性-2连锁的Ss-Slp区域在小鼠补体控制中的作用。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):863-4. doi: 10.1073/pnas.70.3.863.
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Fourth component of human complement: description of a three polypeptide chain structure.人类补体的第四成分：一种三多肽链结构的描述
J Exp Med. 1974 Nov 1;140(5):1324-35. doi: 10.1084/jem.140.5.1324.