Quilichini Yann, Bakhoum Abdoulaye J S, Justine Jean-Lou, Bray Rodney A, Bâ Cheikh T, Marchand Bernard
GEM - Service d'Etude et de Recherche en Microscopie Electronique (SERME), UMR 6134 -SPE, CNRS -University of Corsica , Corte , Corsica , France.
GEM - Service d'Etude et de Recherche en Microscopie Electronique (SERME), UMR 6134 -SPE, CNRS -University of Corsica, Corte, Corsica, France; Laboratory of Evolutionary Biology, Ecology and Management of Ecosystems, University Cheikh Anta Diop of Dakar, Dakar, Senegal.
PeerJ. 2016 Sep 20;4:e2488. doi: 10.7717/peerj.2488. eCollection 2016.
Spermatological characteristics of species from two monorchiid genera, Opisthomonorchis and Paramonorcheides, have been investigated, for the first time, by means of transmission electron microscopy. The ultrastructural study reveals that the mature spermatozoon of Opisthomonorchis dinema and Paramonorcheides selaris share several characters such as the presence of two axonemes of different lengths showing the 9+"1" pattern of the Trepaxonemata, a nucleus, two mitochondria, two bundles of parallel cortical microtubules, external ornamentation of the plasma membrane, spine-like bodies, granules of glycogen and similar morphologies of the anterior and posterior extremities. The slight differences between the male gamete of O. dinema and P. selaris are the length of the first axoneme and the position of the second mitochondrion. This study also elucidates the general morphology of the spermatozoon in all monorchiid species described so far, which corresponds to a unique spermatozoon type. Other interesting finds concern the spermatological similarities between monorchiid spermatozoa and the mature spermatozoon reported in the apocreadiid Neoapocreadium chabaudi. These similarities allow us to suggest a close phylogenetical relationship between the Monorchiidae and the Apocreadiidae, although more studies are needed, especially in the unexplored taxa.
首次通过透射电子显微镜对两个单睾科属（后睾吸虫属和拟后睾吸虫属）物种的精子学特征进行了研究。超微结构研究表明，迪氏后睾吸虫和塞氏拟后睾吸虫的成熟精子具有几个共同特征，例如存在两条不同长度的轴丝，呈现出三鞭毛类的9 + “1”模式、一个细胞核、两个线粒体、两束平行的皮质微管、质膜的外部装饰、棘状体、糖原颗粒以及前后端相似的形态。迪氏后睾吸虫和塞氏拟后睾吸虫雄配子之间的细微差异在于第一轴丝的长度和第二线粒体的位置。这项研究还阐明了迄今为止所描述的所有单睾科物种精子的一般形态，这对应于一种独特的精子类型。其他有趣的发现涉及单睾科精子与报道的阿波克里亚科查氏新阿波克里亚吸虫成熟精子之间的精子学相似性。这些相似性使我们能够提出单睾科和阿波克里亚科之间存在密切的系统发育关系，尽管还需要更多的研究，特别是在未探索的分类群中。
