Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovak Republic,
Parasitol Res. 2014 Jul;113(7):2483-91. doi: 10.1007/s00436-014-3897-6. Epub 2014 Apr 26.
Spermatological characters of the digenean Brandesia turgida (Brandes, 1888), an intestinal parasite of the frog Pelophylax ridibundus (Pallas, 1771), have been investigated by means of transmission electron microscopy for the first time. The process of the spermatozoon formation begins with the appearance of the differentiation zone bordered by cortical microtubules and containing two centrioles associated with striated rootlets and with an intercentriolar body. The intercentriolar body is made up of seven distinct electron-dense plates, two less electron-dense, and four electron-lucent zones. The orthogonal development of the two flagella is followed by a flagellar rotation and their proximodistal fusion with the median cytoplasmic process. This process is accompanied by an extension of both the mitochondrion and nucleus into the median cytoplasmic process. The mature spermatozoon of B. turgida contains two parallel axonemes of unequal lengths with the 9 + "1" trepaxonematan pattern, mitochondrion, nucleus, parallel cortical microtubules, four electron-dense attachment zones, an external ornamentation of the plasma membrane, and electron-dense glycogen granules. The anterior extremity of the male gamete contains one complete centriole, a small component of the central element of the second centriole, and peripheral cortical microtubules (up to 45). The posterior extremity of the mature spematozoon exhibits tubular elements of the disorganized axoneme. The present study provides the first data on spermiogenesis within the family Pleurogenidae. Variations of the spermatozoa ultrastructural characters within Digenea, in particular, between different families of the superfamily Microphalloidea, are discussed.
首次通过透射电子显微镜研究了寄生在青蛙 Pelophylax ridibundus（Pallas，1771）肠道中的吸虫 Brandesia turgida（Brandes，1888）的精子学特征。精子形成过程始于皮层微管包围的分化区的出现，该分化区包含两个与纹状根相连的中心粒和一个中心粒间体。中心粒间体由七个明显的电子致密板、两个电子密度较低的板和四个电子透明区组成。两个鞭毛的正交发育之后是鞭毛的旋转及其与中央细胞质突的近-远融合。这一过程伴随着线粒体和核向中央细胞质突的延伸。B. turgida 的成熟精子包含两个具有 9+“1”三螺旋结构的平行轴丝，线粒体、核、平行的皮层微管、四个电子致密附着区、质膜的外部装饰和电子致密的糖原颗粒。雄性配子的前端包含一个完整的中心粒、第二个中心粒的中央元件的一小部分和周围的皮层微管（多达 45 个）。成熟精子的后端表现出轴丝的管状元素的无序化。本研究提供了 Pleurogenidae 家族内精子发生的第一个数据。讨论了 Digenea 中精子超微结构特征的变化，特别是在 Microphalloidea 超科的不同科之间。
