Department of Biological Sciences, Oakland University, Rochester, Michigan, USA.
OUWB Institute for Stem Cell and Regenerative Medicine, Rochester, Michigan, USA.
J Tissue Eng Regen Med. 2018 Jan;12(1):e579-e591. doi: 10.1002/term.2330. Epub 2017 Mar 22.
Intervertebral disc (IVD) degeneration is characterized by the loss of nucleus pulposus (NP), which is a common cause for lower back pain. Although, currently, there is no cure for the degenerative disc disease, stem cell therapy is increasingly being considered for its treatment. In this study, we investigated the feasibility and efficacy of human umbilical cord mesenchymal stem cells (MSCs) and chondroprogenitor cells (CPCs) derived from those cells to regenerate damaged IVD in a rabbit model. Transplanted cells survived, engrafted and dispersed into NP in situ. Significant improvement in the histology, cellularity, extracellular matrix proteins, and water and glycosaminoglycan contents in IVD recipients of CPCs was observed compared to MSCs. In addition, IVDs receiving CPCs exhibited higher expression of NP-specific human markers, SOX9, aggrecan, collagen 2, FOXF1 and KRT19. The novelty of the study is that in vitro differentiated CPCs derived from umbilical cord MSCs, demonstrated far greater capacity to regenerate damaged IVDs, which provides basis and impetus for stem cell based clinical studies to treat degenerative disc disease. Copyright © 2016 John Wiley & Sons, Ltd.
椎间盘（IVD）退变的特征是髓核（NP）的丢失，这是腰痛的常见原因。尽管目前没有治疗退行性椎间盘疾病的方法，但干细胞疗法越来越被认为是一种可行的治疗方法。在这项研究中，我们研究了人脐带间充质干细胞（MSCs）和软骨祖细胞（CPCs）及其衍生细胞在兔模型中再生受损的 IVD 的可行性和疗效。移植细胞存活、植入并原位分布于 NP 中。与 MSC 相比，CPCs 治疗组的 IVD 组织学、细胞数、细胞外基质蛋白以及水和糖胺聚糖含量均有显著改善。此外，接受 CPCs 的 IVD 表现出更高的 NP 特异性人标志物，SOX9、聚集蛋白聚糖、胶原 2、FOXF1 和 KRT19 的表达。本研究的新颖之处在于，体外分化的源自脐带 MSC 的 CPCs 显示出更大的再生受损 IVD 的能力，这为基于干细胞的临床研究治疗退行性椎间盘疾病提供了基础和动力。版权所有 © 2016 约翰威立父子公司
