Shoda J, Osuga T, Matsuura K, Mahara R, Tohma M, Tanaka N, Matsuzaki Y, Miyazaki H
Institute of Clinical Medicine, University of Tsukuba, Ibaraki, Japan.
J Lipid Res. 1989 Aug;30(8):1233-42.
3 beta-Hydroxy-(delta 5-3 beta-ol), 3 beta,12 alpha-dihydroxy-(delta 5-3 beta,12 alpha-ol), 3 beta,7 alpha-dihydroxy-(delta 5-3 beta,7 alpha-ol) and 3 beta,7 beta-dihydroxy-(delta 5-3 beta,7 beta-ol) 5-cholenoic acids were identified in patients with liver diseases by gas-liquid chromatography-mass spectrometry (GLC-MS). Of these unusual 3 beta-hydroxy-5-en-metabolites, delta 5-3 beta-ol and delta 5-3 beta,12 alpha-ol were found as major components in the urine of patients with liver diseases (cholestasis, liver cirrhosis, chronic hepatitis, acute hepatitis). Other 3 beta-dihydroxy-5-en-metabolites, delta 5-3 beta,7 alpha-ol and delta 5-3 beta,7 beta-ol, were found as minor components in the urine. The levels of delta 5-3 beta-ol and delta 5-3 beta,12 alpha-ol in urine were correlated with their levels in serum, with total bile acids in the urine, and with liver function, implying that the degree of their increment correlated well with the severity of liver diseases. The most abundant amounts of delta 5-3 beta-ol and delta 5-3 beta,12 alpha-ol were found in the urine as sulfate conjugates in comparison with bile, portal and hepatic venous sera, and liver tissue of the patients. The biliary excretion and hepatic extraction of these 3 beta-hydroxy-5-en-unsaturated bile acids were more impaired and inefficient than those of cholic and chenodeoxycholic acids.
通过气液色谱 - 质谱联用（GLC - MS）在肝病患者中鉴定出了3β - 羟基 - （δ5 - 3β - 醇）、3β，12α - 二羟基 - （δ5 - 3β，12α - 醇）、3β，7α - 二羟基 - （δ5 - 3β，7α - 醇）和3β，7β - 二羟基 - （δ5 - 3β，7β - 醇）5 - 胆烯酸。在这些不寻常的3β - 羟基 - 5 - 烯代谢产物中，δ5 - 3β - 醇和δ5 - 3β，12α - 醇被发现是肝病（胆汁淤积、肝硬化、慢性肝炎、急性肝炎）患者尿液中的主要成分。其他3β - 二羟基 - 5 - 烯代谢产物，δ5 - 3β，7α - 醇和δ5 - 3β，7β - 醇，在尿液中是次要成分。尿液中δ5 - 3β - 醇和δ5 - 3β，12α - 醇的水平与它们在血清中的水平、尿液中的总胆汁酸以及肝功能相关，这意味着它们增加的程度与肝病的严重程度密切相关。与患者的胆汁、门静脉和肝静脉血清以及肝组织相比，尿液中发现的δ5 - 3β - 醇和δ5 - 3β，12α - 醇以硫酸酯结合物的形式含量最为丰富。与胆酸和鹅去氧胆酸相比，这些3β - 羟基 - 5 - 烯不饱和胆汁酸的胆汁排泄和肝脏摄取受到的损害更大且效率更低。
