Islam A, Glomski C, Henderson E S
Department of Medical Oncology, Roswell Park Memorial Institute, Buffalo, NY 14263.
J Med. 1989;20(2):113-22.
To study the ability of endosteal cells to spontaneously differentiate in vitro, cells isolated from the endosteal bone surface (endosteal cells) were incubated in liquid suspension cultures containing fetal calf serum and antibiotics, but without chemical inducers of differentiation. Cultures were examined daily for the appearance of morphologically recognizable mature and differentiated hemopoietic cells. Culture outcomes were uniformly consistent, showed an almost complete disappearance of the endosteal cells (which appeared as stimulated lymphoid and late stage normoblast-like cells once they are separated from the bone surfaces), and their replacement by differentiated and mature hemopoietic cells. The ability of endosteal cells to fully differentiate into mature hemopoietic cells in vitro indicates that endosteal cells represent the multipotential hemopoietic stem cells in vivo, and are equivalent of embryonal stage undifferentiated mesenchymal cells.
为研究骨内膜细胞在体外自发分化的能力，从骨内膜骨表面分离得到的细胞（骨内膜细胞）在含有胎牛血清和抗生素但无化学分化诱导剂的液体悬浮培养体系中进行培养。每天检查培养物中形态上可识别的成熟和分化造血细胞的出现情况。培养结果始终一致，显示骨内膜细胞几乎完全消失（这些细胞一旦从骨表面分离出来，表现为受刺激的淋巴细胞和晚期成红细胞样细胞），并被分化和成熟的造血细胞所取代。骨内膜细胞在体外完全分化为成熟造血细胞的能力表明，骨内膜细胞在体内代表多能造血干细胞，等同于胚胎期未分化的间充质细胞。
