Nagata H
J Electron Microsc (Tokyo). 1989;38(2):111-25.
High-voltage electron microscopic observations have been performed on intraglomerular dendritic branches of mitral and tufted cells and spines of granule cells in the Golgi-impregnated mouse olfactory bulb. The observations revealed that intraglomerular tufts of mitral and tufted cells have a very similar structure consisting of four morphologically distinct parts: (i) thick, smoothly outlined proximal parts; (ii) relatively thick varicose parts; (iii) very thin varicose parts, which form terminal branches; and (iv) spine-like small appendages. Granule cell spines in the external plexiform and mitral body layer have terminal swellings of various shapes and sizes, whose width is between 0.4 and 1.5 microns. Spines in the granule cell layer usually have swellings smaller than 0.4 micron in width. In addition, the density of spines per proximal 10 microns length of a deep dendritic trunk was estimated in 10 granule cells and proved to vary greatly from cell to cell--from 7 to 28 spines.
对高尔基浸染的小鼠嗅球中二尖瓣细胞和簇状细胞的肾小球内树突分支以及颗粒细胞的棘进行了高压电子显微镜观察。观察结果显示,二尖瓣细胞和簇状细胞的肾小球内丛状纤维具有非常相似的结构,由四个形态上不同的部分组成:(i)厚的、轮廓光滑的近端部分;(ii)相对较厚的曲张部分;(iii)非常细的曲张部分,形成终末分支;以及(iv)棘状小附属物。外丛状层和二尖瓣体层中的颗粒细胞棘具有各种形状和大小的终末膨大,其宽度在0.4至1.5微米之间。颗粒细胞层中的棘通常具有宽度小于0.4微米的膨大。此外,在10个颗粒细胞中估计了深树突干近端每10微米长度的棘密度,结果证明细胞之间差异很大——从7到28个棘。