Inokuchi T, Yokoyama R, Satoh H, Hamasaki M, Higashi R
J Electron Microsc (Tokyo). 1989;38(3):201-13.
A combined method of corrosion casting and KOH digestion was used for the scanning electron microscopic (SEM) observation of the external walls of the blood vessels running on the surface and in the parenchyma of the rat cerebral cortex. The plastic resin which was preliminarily injected into the cerebral vessels reinforced the preservation of the vessel walls during the chemical digestion. Using the present method, the walls of blood vessels running on the surface and in the parenchyma of the cerebral cortex were clearly seen with SEM. The arterioles showed a rich arrangement of smooth muscle cells embracing the external surface of the endothelia, and the venules had sparse, flat smooth muscle cells. The capillaries were surrounded by many pericytes which were extremely similar to those seen in the other organs of the animals. There were observed two different kinds of pericyte. One type of the pericytes in the present specimens extended very long cytoplasmic processes parallel to the long axis of the capillaries. Another type of the pericytes had relatively short, broad and flat cytoplasmic processes radiated to embrace the capillary walls. The former was frequently seen in the capillaries on brain surface and in the parenchyma, whereas the latter was relatively rare. The availability of the present method was also discussed in comparison with the previous descriptions.
采用腐蚀铸型和氢氧化钾消化相结合的方法，对大鼠大脑皮质表面和实质内的血管外壁进行扫描电子显微镜（SEM）观察。预先注入脑血管的塑料树脂在化学消化过程中增强了血管壁的保存效果。使用本方法，通过SEM可以清晰地看到大脑皮质表面和实质内血管的壁。小动脉显示出丰富的平滑肌细胞排列，环绕在内皮细胞的外表面，而小静脉的平滑肌细胞稀疏且扁平。毛细血管被许多周细胞包围，这些周细胞与在动物其他器官中看到的周细胞极为相似。观察到两种不同类型的周细胞。本标本中的一种周细胞伸出非常长的细胞质突起，与毛细血管的长轴平行。另一种周细胞具有相对短、宽且扁平的细胞质突起，呈放射状环绕毛细血管壁。前者在脑表面和实质内的毛细血管中经常可见，而后者相对较少。还与先前的描述进行了比较，讨论了本方法的实用性。
