Heap R B, Fleet I R, Davis A J, Goode J A, Hamon M H, Walters D E, Flint A P
AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge.
J Endocrinol. 1989 Jul;122(1):147-59. doi: 10.1677/joe.0.1220147.
The mechanisms of lymphatic-vascular transfer across the ovarian vascular pedicle were studied in anaesthetized sheep 8-15 days after ovulation. [3H]Prostaglandin F2 alpha (PGF2 alpha), [14C]mannitol and [36Cl]Na were infused continuously into either a uterine lymphatic or a uterine vein and the kinetics of transfer into the adjacent utero-ovarian vein or ovarian plasma were studied. Transfer occurred according to the sequence [36Cl] greater than [14C] greater than [3H] indicating that PGF2 alpha is not transferred by rapid diffusion, as with [36Cl]Na, nor by a paracellular route, as with [14C]mannitol, but by a slower process probably involving facilitated diffusion. Transfer into the adjacent utero-ovarian vein or ovarian blood was greater when compounds were infused into a uterine lymphatic than into a uterine vein. Substantially more [3H]PGF2 alpha occurred in the adjacent corpus luteum than either of the other compounds after a lymphatic infusion. Intra-lymphatic infusion of PGF2 alpha stimulated the release of ovarian oxytocin but the effect was not confined to the adjacent ovary. Intravenous (jugular) infusion of PGF2 alpha failed to stimulate ovarian oxytocin secretion whereas close-arterial infusion into the ovaries was effective, and the possibility was investigated that any systemic effect of PGF2 alpha was mediated through neural mechanisms. Noradrenaline and acetylcholine were both effective in causing the release of ovarian oxytocin when infused close-arterially into the ovary. With infusions of acetylcholine, ovarian oxytocin secretion rate was increased over fivefold without any change in posterior pituitary release. Noradrenaline and acetylcholine produced a concomitant fall in ovarian blood flow, and neurotransmitter-induced ischaemia may have played a role in ovarian oxytocin release. The finding that PGF2 alpha infused into a uterine lymphatic stimulates ovarian secretion of oxytocin, and that the effect is bilateral whereas PGF2 alpha accumulation in ovarian tissue is unilateral, implies that its mechanism of action may not be solely directed at the luteal cell.
在排卵后8 - 15天的麻醉绵羊中，研究了淋巴血管穿过卵巢血管蒂的机制。将[3H]前列腺素F2α（PGF2α）、[14C]甘露醇和[36Cl]钠持续注入子宫淋巴管或子宫静脉，研究其向相邻子宫卵巢静脉或卵巢血浆转移的动力学。转移顺序为[36Cl]＞[14C]＞[3H]，这表明PGF2α不像[36Cl]钠那样通过快速扩散转移，也不像[14C]甘露醇那样通过细胞旁途径转移，而是通过一个可能涉及易化扩散的较慢过程转移。当化合物注入子宫淋巴管时，向相邻子宫卵巢静脉或卵巢血液的转移比注入子宫静脉时更多。淋巴注入后，相邻黄体中的[3H]PGF2α比其他任何一种化合物都多得多。淋巴内注入PGF2α刺激卵巢催产素释放，但这种作用并不局限于相邻卵巢。静脉（颈静脉）注入PGF2α未能刺激卵巢催产素分泌，而卵巢近动脉注入则有效，并且研究了PGF2α的任何全身作用是否通过神经机制介导的可能性。当近动脉注入卵巢时，去甲肾上腺素和乙酰胆碱都能有效引起卵巢催产素释放。注入乙酰胆碱时，卵巢催产素分泌率增加了五倍多，而后叶垂体释放没有任何变化。去甲肾上腺素和乙酰胆碱导致卵巢血流量同时下降，神经递质诱导的缺血可能在卵巢催产素释放中起作用。注入子宫淋巴管的PGF2α刺激卵巢催产素分泌，且这种作用是双侧的，而PGF2α在卵巢组织中的积累是单侧的，这一发现意味着其作用机制可能不仅仅针对黄体细胞。
