Heap R B, Fleet I R, Hamon M
J Reprod Fertil. 1985 Jul;74(2):645-56. doi: 10.1530/jrf.0.0740645.
[3H]Prostaglandin F-2 alpha (PGF-2 alpha) was infused into a uterine lymphatic vessel or a uterine vein for up to 1 h, or injected into the uterine lumen of anaesthetized non-pregnant sheep 7-15 days after oestrus. After an intraluminal injection, labelled PGF-2 alpha was recovered in uterine lymph and peak radioactivity was reached 50 min after injection. [3H]PGF-2 alpha infused at a constant rate into a uterine lymphatic vessel resulted in a maximum concentration of radioactivity in plasma which was 5.6- and 1.7-fold higher in the adjacent utero-ovarian and ovarian vein, respectively, than in carotid arterial plasma. Estimation of the amount of infusate transferred from a lymphatic into ovarian venous blood gave a value (0.4%) similar to that for transfer from a uterine vein (0.3%). Evidence for local transfer was substantiated by the presence of significantly higher concentrations of 3H-labelled compounds in the ovary and corpus luteum adjacent to the site of intra-lymphatic infusion compared with those in the opposite organs. The concentrations in the adjacent ovary and corpus luteum were significantly greater when an intra-lymphatic rather than intra-uterine vein infusion was adopted. The results show that [3H]PGF-2 alpha is transferred locally from uterine lymphatic vessels into the adjacent ovary, corpus luteum and ovarian vein.
将[3H]前列腺素F-2α（PGF-2α）注入子宫淋巴管或子宫静脉长达1小时，或在发情后7 - 15天注入麻醉的未孕绵羊的子宫腔。腔内注射后，在子宫淋巴液中回收了标记的PGF-2α，注射后50分钟达到放射性峰值。以恒定速率将[3H]PGF-2α注入子宫淋巴管，导致血浆中放射性的最大浓度在相邻的子宫卵巢静脉和卵巢静脉中分别比颈动脉血浆高5.6倍和1.7倍。估计从淋巴管转移到卵巢静脉血中的注入物量的值（0.4%）与从子宫静脉转移的值（0.3%）相似。与对侧器官相比淋巴管内注入部位相邻的卵巢和黄体中3H标记化合物的浓度显著更高，证实了局部转移的证据。当采用淋巴管内注入而非子宫静脉内注入时，相邻卵巢和黄体中的浓度显著更高。结果表明，[3H]PGF-2α从子宫淋巴管局部转移到相邻的卵巢、黄体和卵巢静脉。
