Gu Youfang, Wang Xiaolong, Zhou Cefan, Li Peiying, Xu Qianming, Zhao Changcheng, Liu Wei, Xu Wenlong
J Zoo Wildl Med. 2016 Sep;47(3):846-854. doi: 10.1638/2015-0301.1.
To assess Cryptosporidium infections among wild animals in a zoo located in Anhui province, we conducted an investigation on the fecal samples collected from 44 primates, 41 herbivores, 44 carnivores and omnivores, and 103 birds in the zoo with the use of Sheather's sugar flotation technique and modified acid-fast staining. Cryptosporidium oocysts were detected in the fecal samples from six primates, two herbivores, four carnivores and omnivores, and seven birds by using Sheather's sugar flotation technique; the prevalence of Cryptosporidium infection in primates, herbivores, carnivores and omnivores and birds was 13.64, 4.88, 9.09, and 6.80%, respectively. Modified acid-fast staining detected the presence of Cryptosporidium oocysts in the fecal samples of one primate, three herbivores, 0 carnivores and omnivores, and one bird, and the prevalence of Cryptosporidium infection in primates, herbivores, carnivores and omnivores and birds was 2.27, 7.32, 0.00, and 0.97%, respectively. Polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) and phylogenetic analysis with the use of the neighbor-joining (NJ) method based on the aligned partial small-subunit (SSU) rRNA gene sequences showed that the protozoan pathogen isolated from primates was Cryptosporidium hominis and the pathogen isolated from camels ( Camelus dromedarius ) was Cryptosporidium andersoni. Subtyping the Cryptosporidium hominis by 60-kDa glycoprotein (GP60) gene phylogenetic analysis showed the Cryptosporidium hominis belongs to the subtype IdA and IbA.
为评估安徽省某动物园野生动物隐孢子虫感染情况，我们运用谢尔特氏糖浮选技术和改良抗酸染色法，对该动物园采集的44只灵长类动物、41只食草动物、44只食肉动物和杂食动物以及103只鸟类的粪便样本进行了调查。通过谢尔特氏糖浮选技术在6只灵长类动物、2只食草动物、4只食肉动物和杂食动物以及7只鸟类的粪便样本中检测到隐孢子虫卵囊；灵长类动物、食草动物、食肉动物和杂食动物以及鸟类的隐孢子虫感染率分别为13.64%、4.88%、9.09%和6.80%。改良抗酸染色法在1只灵长类动物、3只食草动物、0只食肉动物和杂食动物以及1只鸟类的粪便样本中检测到隐孢子虫卵囊，灵长类动物、食草动物、食肉动物和杂食动物以及鸟类的隐孢子虫感染率分别为2.27%、7.32%、0.00%和0.97%。基于比对后的部分小亚基（SSU）rRNA基因序列，采用邻接法（NJ）进行聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析和系统发育分析，结果显示从灵长类动物分离出的原生动物病原体为微小隐孢子虫，从骆驼（单峰驼）分离出的病原体为安氏隐孢子虫。通过60 kDa糖蛋白（GP60）基因系统发育分析对微小隐孢子虫进行亚型分析，结果表明微小隐孢子虫属于IdA和IbA亚型。
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