CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal.
Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, Spain; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA), Pamplona, Spain; CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.
Life Sci. 2016 Nov 15;165:100-108. doi: 10.1016/j.lfs.2016.09.023. Epub 2016 Sep 28.
Exercise-stimulated myokine secretion into circulation may be related with browning in white adipose tissue (WAT), representing a positive metabolic effect on whole-body fat mass. However, limited information is yet available regarding the impact of exercise on myokine-related modulation of adipocyte phenotype in WAT from obese rats.
Sprague-Dawley rats (n=60) were divided into sedentary and voluntary physical activity (VPA) groups and fed with standard (35kcal% fat) or high-fat (HFD, 71kcal% fat)-isoenergetic diets. The VPA-groups had unrestricted access to wheel running throughout the protocol. After-9weeks, half of sedentary standard (SS) and sedentary HFD (HS)-fed animals were exercised on treadmill (endurance training, ET) for 8-weeks while maintaining the dietary treatments.
The adipocyte hypertrophy induced by HFD were attenuated by VPA and ET. HFD decreased 5' AMP-activated protein kinase (AMPK) activity in muscle as well as peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and uncoupling protein 1 (UCP1) proteins in eWAT, while not affecting circulating irisin. VPA increased eWAT Tmem26 mRNA levels in the standard diet-fed group, whereas ET increased AMPK, interleukin 6 (IL-6) and fibronectin type III domain-containing protein 5 (FNDC5) protein expression in muscle, but had no impact on circulating irisin protein content. In eWAT, ET increased bone morphogenetic protein 7 (Bmp7), Cidea and PGC-1α in both diet-fed animals, whereas BMP7, Prdm16, UCP1 and FNDC5 only in standard diet-fed group.
Data suggest that ET-induced myokine production seems to contribute, at least in part, to the "brown-like" phenotype in WAT from rats fed a HFD.
运动刺激肌肉因子分泌到血液循环中可能与白色脂肪组织（WAT）中的褐色化有关，这代表对全身脂肪量的积极代谢作用。然而，关于运动对肥胖大鼠 WAT 中脂肪细胞表型的肌肉因子相关调节的影响，信息仍然有限。
将 Sprague-Dawley 大鼠（n=60）分为久坐不动和自愿体力活动（VPA）组，并给予标准（35kcal%脂肪）或高脂肪（HFD，71kcal%脂肪）-等能量饮食。VPA 组在整个方案中可自由使用轮式跑步器。9 周后，一半的久坐不动的标准（SS）和久坐不动的 HFD（HS）喂养动物在跑步机上进行 8 周的耐力训练（ET），同时保持饮食治疗。
HFD 引起的脂肪细胞肥大被 VPA 和 ET 减弱。HFD 降低了肌肉中的 5' AMP 激活蛋白激酶（AMPK）活性以及 eWAT 中的过氧化物酶体增殖物激活受体γ共激活因子 1α（PGC-1α）和解偶联蛋白 1（UCP1）蛋白，但不影响循环中的鸢尾素。VPA 增加了标准饮食喂养组的 eWAT Tmem26 mRNA 水平，而 ET 增加了肌肉中的 AMPK、白细胞介素 6（IL-6）和纤维连接蛋白 III 结构域蛋白 5（FNDC5）蛋白表达，但对循环中的鸢尾素蛋白含量没有影响。在 eWAT 中，ET 增加了两种饮食喂养动物的骨形态发生蛋白 7（Bmp7）、Cidea 和 PGC-1α，而 Bmp7、Prdm16、UCP1 和 FNDC5 仅在标准饮食喂养组中增加。
数据表明，ET 诱导的肌肉因子产生似乎至少部分贡献于 HFD 喂养大鼠的 WAT 中的“褐色样”表型。
