Zhang Yan-Ji, Li Jia, Huang Wei, Mo Guo-Yan, Wang Li-Hua, Zhuo Yue, Zhou Zhong-Yu
College of Acupuncture and Orthopedics, Hubei University of Chinese Medicine, Wuhan 430061, China.
Department of Acupuncture-moxibustion, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061.
Zhen Ci Yan Jiu. 2019 Jul 25;44(7):476-80. doi: 10.13702/j.1000-0607.180460.
To investigate the effect of electroacupuncture (EA) plus treadmill exercise on the expression of peroxisome proliferator activated receptor γ coactivator 1α(PGC-1α), Irisin, AMP-activated protein kinase (AMPK) in skeletal muscle of diet-induced obesity (DIO) rats, so as to explore its mechanism underlying body reduction promotion.
Forty-two male SD rats were divided into normal diet (control, ＝10), high fat diet (model), EA, treadmill exercise and EA plus treadmill exercise (combination) groups (＝8 in each of the latter 4 groups). The obesity model was established by feeding the rats with high fat diet. EA (2 Hz/15 Hz, 1 mA) was applied to bilateral "Zusanli" (ST36) and "Tianshu" (ST25) for 30 min, 5 times per week for a total of 8 weeks. Rats of the treadmill exercise group were forced to perform exercise on a treadmill (16 m/min) for 30 min, 5 times per week for a total of 8 weeks. Rats in the combination group received the above-mentioned two methods. During the treatment, rats in the control group were fed with normal fodder, rats in other groups were fed with high fat fodder, and their body weight was measured once a week. The expression levels of PGC-1α， fibronectin type Ⅲ domain containing 5 (FNDC5), AMPK mRNA and protein of skeletal muscle were measured by quantitative real-time PCR and Western blot，respectively.
After modeling, the body weight was significantly increased (<0.05), and the expression levels of PGC-1α and FNDC5 mRNA and protein, AMPK mRNA and phosphorylated AMPK (p-AMPK) protein in the skeletal muscle were considerably decreased in the model group relevant to the control group (<0.05). Following the treatment, the body weight was significantly down-regulated, while the expression levels of PGC-1α and FNDC5 mRNAs and proteins, AMPK mRNA and p-AMPK protein were obviously up-regulated in the EA, treadmill exercise and combination groups relevant to the model group (<0.05). The therapeutic effect of EA plus treadmill exercise was significantly superior to those of both simple EA and simple treadmill exercise in down-regulating the body weight, as well as in up-regulating the expression of PGC-1α and FNDC5 mRNAs and proteins, AMPK mRNA, and p-AMPK protein (<0.05).
Both EA and treadmill exercise can significantly increase the expression of PGC-1α， FNDC5 and p-AMPK in skeletal muscle of DIO rats, suggesting their efficacy in restoring fatty acid oxidation in skeletal muscle cells and improving mitochondrial function, which may contribute to their function in body reduction. The therapeutic effect of EA plus treadmill exercise is better than that of simple EA and simple treadmill exercise.
探讨电针（EA）联合跑步机运动对饮食诱导肥胖（DIO）大鼠骨骼肌中过氧化物酶体增殖物激活受体γ共激活因子1α（PGC-1α）、鸢尾素、腺苷酸活化蛋白激酶（AMPK）表达的影响，以探讨其促进体重减轻的潜在机制。
将42只雄性SD大鼠分为正常饮食组（对照组，n＝10）、高脂饮食组（模型组）、电针组、跑步机运动组和电针联合跑步机运动组（联合组，后4组每组n＝8）。通过高脂饮食喂养大鼠建立肥胖模型。将电针（2Hz/15Hz，1mA）施加于双侧“足三里”（ST36）和“天枢”（ST25），每次30分钟，每周5次，共8周。跑步机运动组大鼠被迫在跑步机上以16m/min的速度运动30分钟，每周5次，共8周。联合组大鼠接受上述两种方法。治疗期间，对照组大鼠喂以正常饲料，其他组大鼠喂以高脂饲料，每周测量一次体重。分别采用定量实时PCR和蛋白质免疫印迹法检测骨骼肌中PGC-1α、含Ⅲ型纤连蛋白结构域5（FNDC5）、AMPK mRNA和蛋白的表达水平。
建模后，模型组大鼠体重显著增加（P<0.05），与对照组相比，骨骼肌中PGC-1α和FNDC5 mRNA及蛋白、AMPK mRNA和磷酸化AMPK（p-AMPK）蛋白的表达水平显著降低（P<0.05）。治疗后，与模型组相比，电针组、跑步机运动组和联合组大鼠体重显著下调，而骨骼肌中PGC-1α和FNDC5 mRNA及蛋白、AMPK mRNA和p-AMPK蛋白的表达水平明显上调（P<0.05）。在下调体重以及上调PGC-1α和FNDC5 mRNA及蛋白、AMPK mRNA和p-AMPK蛋白的表达方面，电针联合跑步机运动的治疗效果明显优于单纯电针和单纯跑步机运动（P<0.05）。
电针和跑步机运动均可显著增加DIO大鼠骨骼肌中PGC-1α、FNDC5和p-AMPK的表达，提示它们在恢复骨骼肌细胞脂肪酸氧化和改善线粒体功能方面具有功效，这可能有助于它们发挥减轻体重的作用。电针联合跑步机运动的治疗效果优于单纯电针和单纯跑步机运动。
