Carmichael S W, Brooks J C, Malhotra R K, Wakade T D, Wakade A R
Department of Anatomy, Mayo Clinic, Rochester, Minnesota 55905.
J Electron Microsc Tech. 1989 Aug;12(4):316-22. doi: 10.1002/jemt.1060120404.
Evidence is presented for morphological proof of exocytosis in the rat adrenal medulla in situ. Techniques were modified to allow perfusion of the intact adrenal gland with secretagogues (or electrical stimulation) followed by tannic acid. Unstimulated specimens demonstrated exocytotic (omega-shaped) profiles filled with flocculent material. This flocculation was also seen in the intercellular space. Stimulation of the adrenal medulla also resulted in the appearance of exocytotic profiles and an accumulation of the flocculent mass. This was often most evident in the subendothelial space. This is the first demonstration of exocytosis in the rat adrenal medulla by electron microscopy. The techniques used in this study will be useful for studying the pathway of secretory products of the adrenal chromaffin cell before they enter the vascular system.
本文提供了大鼠肾上腺髓质原位胞吐作用的形态学证据。对技术进行了改进，以便在给完整的肾上腺灌注促分泌素（或电刺激）后再用单宁酸处理。未受刺激的标本显示充满絮状物质的胞吐（ω形）轮廓。这种絮凝现象在细胞间隙中也可见到。刺激肾上腺髓质也会导致胞吐轮廓的出现和絮状物质的积累。这在血管内皮间隙中通常最为明显。这是通过电子显微镜首次证明大鼠肾上腺髓质存在胞吐作用。本研究中使用的技术将有助于研究肾上腺嗜铬细胞分泌产物在进入血管系统之前的分泌途径。
