Tosi A L, Campana L G, Dughiero F, Forzan M, Rastrelli M, Sieni E, Rossi C R
Melanoma and Sarcoma Pathology Unit, Veneto Institute of Oncology IOV IRCCS, Via Gattamelata, 64, 35128, Padua, Italy.
Sarcoma and Melanoma Unit, Veneto Institute of Oncology IOV IRCCS, Via Gattamelata, 64, 35128, Padua, Italy.
Med Biol Eng Comput. 2017 Jul;55(7):1097-1108. doi: 10.1007/s11517-016-1573-y. Epub 2016 Oct 1.
Tissue electrical conductivity is correlated with tissue characteristics. In this work, some soft tissue sarcomas (STS) excised from patients have been evaluated in terms of histological characteristics (cell size and density) and electrical resistance. The electrical resistance has been measured using the ex vivo study on soft tissue tumors electrical characteristics (ESTTE) protocol proposed by the authors in order to study electrical resistance of surgical samples excised by patients in a fixed measurement setup. The measurement setup includes a voltage pulse generator (700 V, 100 µs long at 5 kHz, period 200 µs) and an electrode with 7 needles, 20 mm-long, with the same distance arranged in a fixed hexagonal geometry. In the ESTTE protocol, the same voltage pulse sequence is applied to each different tumor mass and the corresponding resistance has been evaluated from voltage and current recorded by the equipment. For each tumor mass, a histological sample of the volume treated by means of voltage pulses has been taken for histological analysis. Each mass has been studied in order to identify the sarcoma type. For each histological sample, an image at 20× or 40× of magnification was acquired. In this work, the electrical resistance measured for each tumor has been correlated with tissue characteristics like the type, size and density of cells. This work presents a preliminary study to explore possible correlations between tissue characteristics and electrical resistance of STS. These results can be helpful to adjust the pulse voltage intensity in order to improve the electrochemotherapy efficacy on some histotype of STS.
组织电导率与组织特征相关。在这项工作中，对从患者身上切除的一些软组织肉瘤（STS）进行了组织学特征（细胞大小和密度）和电阻方面的评估。电阻是使用作者提出的软组织肿瘤电特性离体研究（ESTTE）方案进行测量的，以便在固定测量装置中研究患者切除的手术样本的电阻。测量装置包括一个电压脉冲发生器（700 V，5 kHz时100 µs长，周期200 µs）和一个带有7根20 mm长针的电极，这些针以固定的六边形几何形状等距排列。在ESTTE方案中，将相同的电压脉冲序列应用于每个不同的肿瘤块，并根据设备记录的电压和电流评估相应的电阻。对于每个肿瘤块，取通过电压脉冲处理的体积的组织学样本进行组织学分析。对每个肿块进行研究以确定肉瘤类型。对于每个组织学样本，获取20倍或40倍放大倍数的图像。在这项工作中，测量的每个肿瘤的电阻已与细胞类型、大小和密度等组织特征相关联。这项工作提出了一项初步研究，以探索组织特征与STS电阻之间可能的相关性。这些结果有助于调整脉冲电压强度，以提高对某些组织类型的STS的电化学治疗效果。
