Department of Electrical Engineering, State University of Santa Catarina, Joinville-SC, Brazil.
IEEE Trans Biomed Eng. 2012 Oct;59(10):2965-73. doi: 10.1109/TBME.2012.2212896.
Conductivity measurements in suspensions of biological cells have been used since many years for electroporation effectiveness evaluation. However, conductivity modeling by means of instantaneous values of current and voltage during pulse application does not take into account the effects of the sample reactance and the dielectric dispersion of the medium. This can lead to misinterpretation in the electroporation analysis. The electrical modeling and characterization of electroporation using sinusoidal signal analysis at 10 kHz proposed in this paper allows us to avoid distortions due to reactive effects of the sample. A simple equation establishes the relation between suspension conductivity and membrane conductance. This model was used in experiments with suspensions of yeast cells and applied electric fields of up to 450 kV/m for 1 ms. The analysis using the proposed model resulted in membrane conductance values of up to 8000 S/m (2) and allowed estimating the distribution profile of conductance on the cell membrane.
多年来，生物细胞悬浮液的电导率测量一直被用于评估电穿孔的效果。然而，通过在施加脉冲时的电流和电压的瞬时值来对电导率建模，并不能考虑到样品电抗和介质色散的影响。这可能导致在电穿孔分析中出现错误的解释。本文提出的使用 10 kHz 正弦信号分析进行电穿孔的电气建模和特性分析，可以避免由于样品的反应性而产生的失真。一个简单的公式建立了悬浮液电导率和细胞膜电导率之间的关系。该模型应用于酵母细胞悬浮液的实验中，施加的电场高达 450 kV/m，持续时间为 1 ms。使用所提出的模型进行分析，得到的细胞膜电导率值高达 8000 S/m(2)，并允许估计细胞膜上电导的分布轮廓。
