Bäcker Anne, Erhardt Olga, Wietbrock Lukas, Schel Natalia, Göppert Bettina, Dirschka Marian, Abaffy Paul, Sollich Thomas, Cecilia Angelica, Gruhl Friederike J
Karlsruhe Institute of Technology (KIT), Institute Microstructure Technology (IMT), Eggenstein-Leopoldshafen, 76344, Germany.
Karlsruhe Institute of Technology (KIT), Institute of Functional Interfaces (IFG), Eggenstein-Leopoldshafen, 76344, Germany.
Biopolymers. 2017 Feb;107(2):70-79. doi: 10.1002/bip.22993.
In the present work, different biopolymer blend scaffolds based on the silk protein fibroin from Bombyx mori (BM) were prepared via freeze-drying method. The chemical, structural, and mechanical properties of the three dimensional (3D) porous silk fibroin (SF) composite scaffolds of gelatin, collagen, and chitosan as well as SF from Antheraea pernyi (AP) and the recombinant spider silk protein spidroin (SSP1) have been systematically investigated, followed by cell culture experiments with epithelial prostate cancer cells (LNCaP) up to 14 days. Compared to the pure SF scaffold of BM, the blend scaffolds differ in porous morphology, elasticity, swelling behavior, and biochemical composition. The new composite scaffold with SSP1 showed an increased swelling degree and soft tissue like elastic properties. Whereas, in vitro cultivation of LNCaP cells demonstrated an increased growth behavior and spheroid formation within chitosan blended scaffolds based on its remarkable porosity, which supports nutrient supply matrix. Results of this study suggest that silk fibroin matrices are sufficient and certain SF composite scaffolds even improve 3D cell cultivation for prostate cancer research compared to matrices based on pure biomaterials or synthetic polymers.
在本研究中，通过冷冻干燥法制备了基于家蚕（BM）丝蛋白纤维蛋白的不同生物聚合物共混支架。对明胶、胶原蛋白和壳聚糖以及柞蚕（AP）丝素蛋白（SF）和重组蜘蛛丝蛋白（SSP1）的三维（3D）多孔丝素蛋白（SF）复合支架的化学、结构和力学性能进行了系统研究，随后对上皮性前列腺癌细胞（LNCaP）进行了长达14天的细胞培养实验。与BM的纯SF支架相比，共混支架在多孔形态、弹性、溶胀行为和生化组成方面存在差异。含有SSP1的新型复合支架显示出更高的溶胀度和类似软组织的弹性性能。而在体外培养LNCaP细胞时，基于壳聚糖共混支架显著的孔隙率，其生长行为增加且形成了球体，这有助于营养供应基质。本研究结果表明，丝素蛋白基质是足够的，与基于纯生物材料或合成聚合物的基质相比，某些SF复合支架甚至能改善前列腺癌研究中的3D细胞培养。
