Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049, Madrid, Spain.
Microb Biotechnol. 2017 Nov;10(6):1457-1467. doi: 10.1111/1751-7915.12422. Epub 2016 Oct 3.
Laccases are multicopper containing enzymes capable of performing one electron oxidation of a broad range of substrates. Using molecular oxygen as the final electron acceptor, they release only water as a by-product, and as such, laccases are eco-friendly, versatile biocatalysts that have generated an enormous biotechnological interest. Indeed, this group of enzymes has been used in different industrial fields for very diverse purposes, from food additive and beverage processing to biomedical diagnosis, and as cross-linking agents for furniture construction or in the production of biofuels. Laccases have also been studied intensely in nanobiotechnology for the development of implantable biosensors and biofuel cells. Moreover, their capacity to transform complex xenobiotics makes them useful biocatalysts in enzymatic bioremediation. This review summarizes the most significant recent advances in the use of laccases and their future perspectives in biotechnology.
漆酶是一类多铜含酶，能够对广泛的底物进行单电子氧化。它们利用分子氧作为最终电子受体，只释放水作为副产物，因此，漆酶是环保、多功能的生物催化剂，引起了巨大的生物技术兴趣。事实上，这组酶已经在不同的工业领域用于非常多样化的目的，从食品添加剂和饮料加工到生物医学诊断，以及作为家具制造或生物燃料生产中的交联剂。漆酶也在纳米生物技术中得到了深入研究，用于开发可植入生物传感器和生物燃料电池。此外，它们转化复杂异源生物的能力使它们成为酶法生物修复中的有用生物催化剂。本综述总结了漆酶在生物技术中应用的最新重要进展及其未来展望。
