Huang Yaoxin, Ye Mu, Cao Xinang, Chen Haiqiang
Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716-2150, USA.
Department of Animal and Food Sciences, University of Delaware, Newark, DE 19716-2150, USA.
Food Microbiol. 2017 Feb;61:1-4. doi: 10.1016/j.fm.2016.08.001. Epub 2016 Aug 2.
Pulsed light (PL) inactivation of two human norovirus (HuNoV) surrogates, murine norovirus (MNV-1) and Tulane virus (TV), and two bacterial pathogens, Escherichia coli O157:H7 and Salmonella, were evaluated. The viruses and bacteria were suspended in phosphate buffered saline (PBS) to final populations of ∼6 log PFU/mL and ∼6 log CFU/mL, respectively. Both viral and bacterial suspensions were then irradiated by PL for different durations and the reductions of each microorganisms were determined. MNV-1 and TV were significantly (P < 0.05) more resistant to PL treatment than Salmonella and E. coli O157:H7 in PBS suspension. MNV-1, Salmonella and E. coli O157:H7 were also inoculated on strawberries and blueberries and the PL inactivation of each microorganism was determined. Lower inactivation of each microorganism was achieved on berry surfaces than in PBS suspension. This study shows that PL can induce rapid inactivation of MNV-1, TV, Salmonella and E. coli O157:H7 in clear suspension with viruses more resistant to PL treatment than bacteria. The efficacy of PL treatment is substantially influenced by food surface structure.
评估了脉冲光（PL）对两种人类诺如病毒（HuNoV）替代物，即小鼠诺如病毒（MNV-1）和图兰病毒（TV），以及两种细菌病原体，即大肠杆菌O157:H7和沙门氏菌的灭活效果。将病毒和细菌分别悬浮于磷酸盐缓冲盐水（PBS）中，使其最终浓度分别达到约6 log PFU/mL和约6 log CFU/mL。然后对病毒和细菌悬液进行不同时长的PL照射，并测定每种微生物的减少量。在PBS悬液中，MNV-1和TV对PL处理的抗性显著（P < 0.05）高于沙门氏菌和大肠杆菌O157:H7。还将MNV-1、沙门氏菌和大肠杆菌O157:H7接种到草莓和蓝莓上，并测定每种微生物的PL灭活情况。在浆果表面，每种微生物的灭活程度低于在PBS悬液中的灭活程度。本研究表明，PL可使清悬液中的MNV-1、TV、沙门氏菌和大肠杆菌O157:H7快速灭活，其中病毒对PL处理的抗性高于细菌。PL处理的效果受食品表面结构的显著影响。
