Institute of Nanoscience of Aragon (INA) and Department of Chemical, Engineering and Environmental Technology, University of Zaragoza, C/ Mariano Esquillor, s/n, I+D+i Building, 50018 Zaragoza, Spain; LAQV, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Institute of Nanoscience of Aragon (INA) and Department of Chemical, Engineering and Environmental Technology, University of Zaragoza, C/ Mariano Esquillor, s/n, I+D+i Building, 50018 Zaragoza, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Centro de Investigación Biomédica en Red. C/ Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain.
J Colloid Interface Sci. 2017 Jan 15;486:144-152. doi: 10.1016/j.jcis.2016.09.059. Epub 2016 Sep 28.
In this work, tris(phenantroline)ruthenium(II) chloride (Ru(phen)) was immobilized in silica nanoparticles prepared according to the Stöber method. Efforts were devoted on the optimization of the nano-thermometer in terms of size, polydispersity, intensity of the emission and temperature sensitivity. In particular, the immobilization of the luminophore in an external thin shell made of silica grown in a second step on bare silica nanoparticles allowed producing fluorescent monodisperse silica nanoparticles (420±20nm). A systematic study was addressed to maximize the intensity of the emission of the fluorescent nanoparticles by adjusting the concentration of Ru(phen) in the shell from 0.2 to 24wt.%, whereas the thickness of the shell is affected by the amount of silica precursor employed. The luminescent activity of the doped nanoparticles was found to be sensitive to the temperature. In fact, the intensity of the emission linearly decreased by increasing the temperature from 20°C to 65°C. The thermoresponsive nanoparticles were functionalized with long aliphatic chains in order to obtain hydrophobic nanoparticles. The developed nanoparticles were immobilized via dip-coating procedure on the surface of hydrophobic porous membranes, such as Polyvinylidene fluoride (PVDF) prepared via Non-Solvent Induced Phase Separation (NIPS), providing local information about the membrane surface temperature.
在这项工作中，三（邻菲咯啉）钌（II）氯化物（Ru（phen））被固定在根据 Stöber 法制备的硅纳米粒子中。我们致力于优化纳米温度计的尺寸、多分散性、发光强度和温度灵敏度。特别是，通过在裸硅纳米粒子上生长的第二步中生长的外部薄壳将发光体固定在其中，从而可以制备荧光单分散硅纳米粒子（420±20nm）。通过调整壳中 Ru（phen）的浓度（从 0.2 到 24wt.%），系统地研究了如何最大程度地提高荧光纳米粒子的发光强度，而壳的厚度则受所用的硅前体的量的影响。掺杂纳米粒子的发光活性对温度敏感。实际上，随着温度从 20°C 增加到 65°C，发光强度呈线性下降。功能化的温敏纳米粒子具有长的脂肪链，以获得疏水性纳米粒子。通过浸涂法将开发的纳米粒子固定在疏水性多孔膜（例如通过非溶剂诱导相分离（NIPS）制备的聚偏二氟乙烯（PVDF））的表面上，从而提供了有关膜表面温度的局部信息。
