Michaud A, Tordjman J, Pelletier M, Liu Y, Laforest S, Noël S, Le Naour G, Bouchard C, Clément K, Tchernof A
Department of Endocrinology and Nephrology, CHU de Quebec-Laval University, Quebec City, Québec, Canada.
School of Nutrition, Laval University, Quebec City, Québec, Canada.
Int J Obes (Lond). 2016 Dec;40(12):1823-1831. doi: 10.1038/ijo.2016.173. Epub 2016 Oct 4.
Adipose tissue fibrosis is a relatively new notion and its relationship with visceral obesity and cardiometabolic alterations remains unclear, particularly in moderate obesity.
Our objective was to examine if total and pericellular collagen accumulation are relevant for the pathophysiology of visceral obesity and related cardiometabolic risk.
Surgical omental (OM) and subcutaneous (SC) fat samples were obtained in 56 women (age: 47.2±5.8 years; body mass index (BMI): 27.1±4.4 kg/m). Body composition and fat distribution were measured by dual-energy X-ray absorptiometry and computed tomography, respectively. Total and pericellular collagen were measured using picrosirius red staining. CD68+ cells (total macrophages) and CD163+ cells (M2-macrophages) were identified using immunohistochemistry.
We found that only pericellular collagen percentage, especially in OM fat, was associated with higher BMI, body fat mass and adipose tissue areas as well as lower radiologic attenuation of visceral adipose tissue and altered cardiometabolic risk variables. Strong correlations between peri-adipocyte collagen percentage and total or M2-macrophage percentages were observed in both depots. Total collagen percentage in either compartment was not related to adiposity, fat distribution or cardiometabolic risk.
As opposed to whole tissue-based assessments of adipose tissue fibrosis, collagen deposition around the adipocyte, especially in the OM fat compartment is related to total and regional adiposity as well as altered cardiometabolic risk profile.
脂肪组织纤维化是一个相对较新的概念，其与内脏肥胖和心脏代谢改变之间的关系仍不明确，尤其是在中度肥胖患者中。
我们的目的是研究总的和细胞周围的胶原蛋白积累是否与内脏肥胖的病理生理学及相关心脏代谢风险有关。
对56名女性（年龄：47.2±5.8岁；体重指数（BMI）：27.1±4.4kg/m²）获取手术切除的网膜（OM）和皮下（SC）脂肪样本。分别采用双能X线吸收法和计算机断层扫描测量身体成分和脂肪分布。使用天狼星红染色法测量总的和细胞周围的胶原蛋白。采用免疫组织化学法鉴定CD68+细胞（总巨噬细胞）和CD163+细胞（M2巨噬细胞）。
我们发现，只有细胞周围胶原蛋白百分比，尤其是在网膜脂肪中，与较高的BMI、体脂量和脂肪组织面积以及较低的内脏脂肪组织放射学衰减和改变的心脏代谢风险变量相关。在两个部位均观察到脂肪细胞周围胶原蛋白百分比与总巨噬细胞或M2巨噬细胞百分比之间存在强相关性。两个部位的总胶原蛋白百分比均与肥胖、脂肪分布或心脏代谢风险无关。
与基于整个组织的脂肪组织纤维化评估不同，脂肪细胞周围的胶原蛋白沉积，尤其是在网膜脂肪部位，与总体和局部肥胖以及改变的心脏代谢风险谱相关。
