CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Sci Rep. 2016 Oct 4;6:34718. doi: 10.1038/srep34718.
A facile "one-pot" approach to prepare organo-silica hybrid capillary monolithic column with intact mesoporous silica nanoparticle (IMSN) as crosslinker and building block was described. An IMSN crosslinked octadecyl-silica hybrid capillary monolithic column (IMSN-C18 monolithic column) was successfully prepared, and the effects of fabrication conditions (e.g. concentration of intact mesoporous silica nanoparticle, polycondensation temperature, content of vinyltrimethoxysilane and stearyl methacrylate) on the structures of the IMSN-C18 monolithic column were studied in detail. The IMSN-C18 hybrid monolithic column possessed uniform morphology, good mechanical and pH stability (pH 1.1-11), which was applied to the separations of alkyl benzenes, polycyclic aromatic hydrocarbons (PAHs), as well as proteins. The minimum plate height of 10.5 μm (corresponding to 95000 N m) for butylbenzene and high reproducibility were achieved. The analysis of tryptic digest of bovine serum albumin (BSA) was carried out on the IMSN-C18 monolithic column by cLC coupled mass spectrometry (cLC-MS/MS), with the protein sequence coverage of 87.5% for BSA, demonstrating its potential application in proteomics.
一种简便的“一锅法”制备有机-硅杂化毛细管整体柱的方法，其中完整的介孔硅纳米颗粒（IMSN）作为交联剂和构建块。成功制备了 IMSN 交联的十八烷基-硅杂化毛细管整体柱（IMSN-C18 整体柱），并详细研究了制备条件（例如完整介孔硅纳米颗粒的浓度、缩聚温度、乙烯基三甲氧基硅烷和硬脂基甲基丙烯酸酯的含量）对 IMSN-C18 整体柱结构的影响。IMSN-C18 杂化整体柱具有均匀的形态、良好的机械和 pH 稳定性（pH 1.1-11），并应用于烷基苯、多环芳烃（PAHs）以及蛋白质的分离。实现了对正丁基苯的 10.5μm 最小板高（相当于 95000N m）和高重现性。通过 cLC-MS/MS 在 IMSN-C18 整体柱上对牛血清白蛋白（BSA）的胰蛋白酶消化物进行了分析，BSA 的蛋白序列覆盖率为 87.5%，表明其在蛋白质组学中的潜在应用。
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