Department of Analytical Chemistry, China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, 210009, People's Republic of China.
Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 210009, People's Republic of China.
Mikrochim Acta. 2019 Mar 15;186(4):244. doi: 10.1007/s00604-019-3318-8.
Magnetic nanoparticles (MNPs) modified with β-cyclodextrin and mono-6-deoxy-6-(1-methylimidazolium)-β-cyclodextrin tosylate (an ionic liquid), which called MNP-β-CD and MNP-β-CD-IL, were coated into the capillary inner wall. Compared to an uncoated capillary, the new systems show good reproducibility and durability. The systems based on the use of MNP-β-CD or MNP-β-CD-IL as stationary phases were established for enantioseparation of Dns-modified amino acids. Improved resolutions were obtained for both CEC systems. Primary parameters such as running buffer pH value and applied voltage were systematically optimized in order to obtain optimal enantioseparations. Under the optimized conditions, the capillaries exhibited excellent chiral recognition ability for six Dns-amino acids (the DL-forms of alanine, leucine, lsoleucine, valine, methionine, glutamic acid) and provided a promising way for the preparation of chiral column. Graphical Abstract Schematic presentation of the open-tubular capillary electrochromatography systems with MNP-β-CD and MNP-β-CD-IL as stationary phases for enantioseparation of dansylated amino acids.
用β-环糊精和单 6-去氧-6-（1-甲基咪唑基）-β-环糊精对甲苯磺酸酯（离子液体）修饰的磁性纳米粒子（MNPs）被涂覆到毛细管内壁。与未涂层的毛细管相比，新系统表现出良好的重现性和耐用性。建立了基于使用 MNP-β-CD 或 MNP-β-CD-IL 作为固定相的手性分离体系，用于拆分 Dns 修饰的氨基酸。两种 CEC 体系都获得了更好的分辨率。为了获得最佳的手性分离，系统地优化了运行缓冲液 pH 值和施加电压等主要参数。在优化条件下，毛细管对六种 Dns-氨基酸（丙氨酸、亮氨酸、异亮氨酸、缬氨酸、蛋氨酸、谷氨酸的 DL 形式）表现出优异的手性识别能力，为手性柱的制备提供了一种有前途的方法。
