CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Anal Bioanal Chem. 2013 Mar;405(7):2265-71. doi: 10.1007/s00216-012-6589-z. Epub 2012 Dec 11.
A butyl-silica hybrid monolithic column for bioseparation by capillary liquid chromatography (cLC) was prepared with butyl methacrylate and alkoxysilanes through a "one-pot" process. The effects of polycondensation temperature, volume percentage of N,N'-dimethylformamide, and content of cetyltrimethylammonium bromide and butyl methacrylate on the morphologies of the hybrid monolithic columns prepared were investigated in detail. Baseline separations of proteins and small peptides on the hybrid monolithic column were achieved by cLC with gradient elution. In addition, the resulting hybrid column was also applied for analysis of tryptic digests of bovine serum albumin by cLC coupled with tandem mass spectrometry. The results demonstrate its potential application in separation of complex biological samples.
一种用于毛细管液相色谱（cLC）生物分离的丁基-硅杂化整体柱是通过“一锅法”过程用甲基丙烯酸丁酯和烷氧基硅烷制备的。详细研究了缩聚温度、N,N'-二甲基甲酰胺体积百分比、十六烷基三甲基溴化铵和甲基丙烯酸丁酯含量对制备的杂化整体柱形态的影响。通过梯度洗脱，在杂化整体柱上实现了蛋白质和小肽的基线分离。此外，所得杂化柱还应用于通过与串联质谱法耦合的 cLC 分析牛血清白蛋白的胰蛋白酶消化物。结果表明其在分离复杂生物样品方面具有潜在的应用。
