Güler Necmettin
Department of Biology, Faculty of Sciences, Trakya University, 22030 Edirne, Turkey.
PhytoKeys. 2016 Aug 2(68):9-25. doi: 10.3897/phytokeys.68.8746. eCollection 2016.
In this study, the seed micromorphologies of eight taxa of , and growing around Edirne province (Turkey) were investigated using light microscopy and scanning electron microscopy (SEM). Slides prepared with glycerin jelly were used for measurements under the light microscope and fine details of seed testae characteristics were observed with SEM. Seeds of the investigated orchid taxa are fusiform shaped and of different shades of brown. Their lengths and widths are different among the taxa and range between 0.263-0.640 mm and 0.118-0.208 mm, respectively. Testa surfaces of Orchis mascula subsp. mascula, Orchis purpurea subsp. purpurea and Orchis simia subsp. simia, are smooth while those of , Anacamptis laxiflora subsp. laxiflora, Anacamptis morio subsp. morio, and Neotinea tridentata subsp. tridentata are reticulate. An identification key based on seed morphologies and sizes is suggested for the first time, including testae structures of orchids growing in Edirne province. The overall results of the study showed that morphological structures of orchid's seeds could be used as diagnostic characters in identification.
在本研究中，利用光学显微镜和扫描电子显微镜（SEM）对生长在土耳其埃迪尔内省周边的8个红门兰属、舌唇兰属和鸟巢兰属分类群的种子微观形态进行了研究。用甘油凝胶制备的载玻片用于在光学显微镜下进行测量，并通过扫描电子显微镜观察种子种皮特征的细微细节。所研究的兰花分类群的种子呈纺锤形，颜色为深浅不一的棕色。它们的长度和宽度在不同分类群之间有所不同，分别在0.263 - 0.640毫米和0.118 - 0.208毫米之间。雄性红门兰亚种、紫花红门兰亚种和无距红门兰亚种的种皮表面光滑，而宽花火烧兰亚种、绿花火烧兰亚种、高斑叶兰和三叉新须兰的种皮表面呈网状。首次提出了基于种子形态和大小的鉴定检索表，包括埃迪尔内省生长的兰花的种皮结构。该研究的总体结果表明，兰花种子的形态结构可作为鉴定中的诊断特征。
