微小RNA-129-5p通过靶向Wnt5a抑制血管平滑肌细胞增殖。
MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a.
作者信息
Zhang Yiming, Liu Zhao, Zhou Min, Liu Changjian
机构信息
Department of Vascular Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu 210008, P.R. China.
出版信息
Exp Ther Med. 2016 Oct;12(4):2651-2656. doi: 10.3892/etm.2016.3672. Epub 2016 Sep 6.
Abstract
Aberrant smooth muscle cells (SMCs) play important roles in the formation of abdominal aortic aneurysm (AAA). Although the molecular mechanism of AAA formation has been investigated, there is a lack of understanding concerning the role of microRNAs (miRNAs) in AAA, which the current study aimed to address. Firstly, miRNA array analysis was performed in order to compare the miRNA profiles in a mouse model of AAA with those in normal control mice, and differentially expressed miRNAs were identified. miR-129-5p was selected for further analysis, and was used to transfect human SMCs. The results of an MTT assay revealed that miR-129-5p inhibited the proliferation of SMCs, and flow cytometry indicated that apoptosis was induced. Bioinformatic analysis predicted that Wnt5a was the potential target gene of miR-129-5p, and this was verified by luciferase assay. In summary, miR-129-5p inhibits cellular proliferation, induces apoptosis and modulates the Wnt5a signaling pathway in SMCs.
摘要
异常平滑肌细胞（SMC）在腹主动脉瘤（AAA）形成过程中发挥重要作用。尽管已经对AAA形成的分子机制进行了研究，但对于微小RNA（miRNA）在AAA中的作用仍缺乏了解，而本研究旨在解决这一问题。首先，进行了miRNA阵列分析，以比较AAA小鼠模型与正常对照小鼠的miRNA谱，并鉴定出差异表达的miRNA。选择miR-129-5p进行进一步分析，并用于转染人SMC。MTT试验结果显示，miR-129-5p抑制SMC的增殖，流式细胞术表明其诱导了细胞凋亡。生物信息学分析预测Wnt5a是miR-129-5p的潜在靶基因，荧光素酶试验验证了这一点。总之，miR-129-5p抑制细胞增殖，诱导细胞凋亡并调节SMC中的Wnt5a信号通路。
相似文献
1
MicroRNA-129-5p inhibits vascular smooth muscle cell proliferation by targeting Wnt5a.微小RNA-129-5p通过靶向Wnt5a抑制血管平滑肌细胞增殖。
Exp Ther Med. 2016 Oct;12(4):2651-2656. doi: 10.3892/etm.2016.3672. Epub 2016 Sep 6.
2
hsa-miR-15b-5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis.人源微小RNA-15b-5p通过靶向ACSS2/PTGS2轴调控人血管平滑肌细胞的增殖和凋亡。
Exp Ther Med. 2021 Nov;22(5):1208. doi: 10.3892/etm.2021.10642. Epub 2021 Aug 24.
3
Abdominal Aortic Aneurysm-Associated MicroRNA-516a-5p Regulates Expressions of Methylenetetrahydrofolate Reductase, Matrix Metalloproteinase-2, and Tissue Inhibitor of Matrix Metalloproteinase-1 in Human Abdominal Aortic Vascular Smooth Muscle Cells.腹主动脉瘤相关的微小RNA-516a-5p调节人腹主动脉血管平滑肌细胞中甲基四氢叶酸还原酶、基质金属蛋白酶-2和基质金属蛋白酶-1组织抑制剂的表达。
Ann Vasc Surg. 2017 Jul;42:263-273. doi: 10.1016/j.avsg.2016.10.062. Epub 2017 Mar 10.
4
LUCAT1 contributes to MYRF-dependent smooth muscle cell apoptosis and may facilitate aneurysm formation via the sequestration of miR-199a-5p.LUCAT1 通过隔离 miR-199a-5p 促进 MYRF 依赖性平滑肌细胞凋亡，并可能通过这种方式促进动脉瘤形成。
Cell Biol Int. 2020 Mar;44(3):755-763. doi: 10.1002/cbin.11270. Epub 2019 Dec 26.
5
Upregulation of MicroRNA-15a Contributes to Pathogenesis of Abdominal Aortic Aneurysm (AAA) by Modulating the Expression of Cyclin-Dependent Kinase Inhibitor 2B (CDKN2B).微小RNA-15a的上调通过调节细胞周期蛋白依赖性激酶抑制剂2B（CDKN2B）的表达促进腹主动脉瘤（AAA）的发病机制。
Med Sci Monit. 2017 Feb 18;23:881-888. doi: 10.12659/msm.898233.
6
miR-155-5p inhibits the viability of vascular smooth muscle cell via targeting FOS and ZIC3 to promote aneurysm formation.miR-155-5p 通过靶向 FOS 和 ZIC3 抑制血管平滑肌细胞的活力，从而促进动脉瘤的形成。
Eur J Pharmacol. 2019 Jun 15;853:145-152. doi: 10.1016/j.ejphar.2019.03.030. Epub 2019 Mar 19.
7
Salidroside inhibits chronic myeloid leukemia cell proliferation and induces apoptosis by regulating the miR-140-5p/wnt5a/β-catenin axis.红景天苷通过调节miR-140-5p/wnt5a/β-连环蛋白轴抑制慢性粒细胞白血病细胞增殖并诱导其凋亡。
Exp Ther Med. 2021 Nov;22(5):1249. doi: 10.3892/etm.2021.10684. Epub 2021 Sep 2.
8
miR-129-5p targets Wnt5a to block PKC/ERK/NF-κB and JNK pathways in glioblastoma.miR-129-5p 通过靶向 Wnt5a 抑制胶质母细胞瘤中的 PKC/ERK/NF-κB 和 JNK 通路。
Cell Death Dis. 2018 Mar 12;9(3):394. doi: 10.1038/s41419-018-0343-1.
9
Inhibition of microRNA-384-5p alleviates osteoarthritis through its effects on inhibiting apoptosis of cartilage cells via the NF-κB signaling pathway by targeting SOX9.miR-384-5p 通过靶向 SOX9 抑制 NF-κB 信号通路抑制软骨细胞凋亡从而缓解骨关节炎。
Cancer Gene Ther. 2018 Dec;25(11-12):326-338. doi: 10.1038/s41417-018-0029-y. Epub 2018 Jul 30.
10
miR-96-5p Regulates Proliferation, Migration, and Apoptosis of Vascular Smooth Muscle Cell Induced by Angiotensin II via Targeting NFAT5.微小RNA-96-5p通过靶向核因子活化T细胞5调控血管紧张素II诱导的血管平滑肌细胞增殖、迁移和凋亡。
J Vasc Res. 2020;57(2):86-96. doi: 10.1159/000505457. Epub 2020 Feb 11.
引用本文的文献
1
Integrated MicroRNA-mRNA Analyses of the Osteogenic Differentiation of Human Dental Pulp Stem Cells by a Helioxanthin Derivative.一种海胆黄质衍生物对人牙髓干细胞成骨分化的MicroRNA-mRNA综合分析
Curr Issues Mol Biol. 2024 Sep 28;46(10):10960-10968. doi: 10.3390/cimb46100651.
2
Roles and Mechanisms of miRNAs in Abdominal Aortic Aneurysm: Signaling Pathways and Clinical Insights.miRNAs 在腹主动脉瘤中的作用和机制：信号通路和临床见解。
Curr Atheroscler Rep. 2024 Jul;26(7):273-287. doi: 10.1007/s11883-024-01204-8. Epub 2024 May 6.
3
Pathogenic role of microRNAs in atherosclerotic ischemic stroke: Implications for diagnosis and therapy.微小RNA在动脉粥样硬化性缺血性卒中中的致病作用：对诊断和治疗的启示。
Genes Dis. 2021 Jan 12;9(3):682-696. doi: 10.1016/j.gendis.2021.01.001. eCollection 2022 May.
4
The role of vascular smooth muscle cells in the development of aortic aneurysms and dissections.血管平滑肌细胞在主动脉瘤和夹层形成中的作用。
Eur J Clin Invest. 2022 Apr;52(4):e13697. doi: 10.1111/eci.13697. Epub 2021 Nov 21.
5
Programmed cell death in aortic aneurysm and dissection: A potential therapeutic target.程序性细胞死亡在主动脉瘤和夹层中的作用：一个潜在的治疗靶点。
J Mol Cell Cardiol. 2022 Feb;163:67-80. doi: 10.1016/j.yjmcc.2021.09.010. Epub 2021 Sep 28.
6
hsa-miR-15b-5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis.人源微小RNA-15b-5p通过靶向ACSS2/PTGS2轴调控人血管平滑肌细胞的增殖和凋亡。
Exp Ther Med. 2021 Nov;22(5):1208. doi: 10.3892/etm.2021.10642. Epub 2021 Aug 24.
7
Aerobic exercise modulates noncoding RNA network upstream of FNDC5 in the Gastrocnemius muscle of high-fat-diet-induced obese mice.有氧运动调节高脂饮食诱导的肥胖小鼠腓肠肌中FNDC5上游的非编码RNA网络。
J Physiol Biochem. 2021 Nov;77(4):589-600. doi: 10.1007/s13105-021-00825-w. Epub 2021 Aug 18.
8
Celastrol ameliorates vascular neointimal hyperplasia through Wnt5a-involved autophagy.藜芦醇通过 Wnt5a 相关自噬减轻血管新生内膜增生。
Int J Biol Sci. 2021 Jun 22;17(10):2561-2575. doi: 10.7150/ijbs.58715. eCollection 2021.
9
miR-140-5p regulates vascular smooth muscle cell viability, migration and apoptosis by targeting ROBO4 gene expression in atherosclerosis.miR-140-5p 通过靶向动脉粥样硬化中的 ROBO4 基因表达来调节血管平滑肌细胞的活力、迁移和凋亡。
Mol Med Rep. 2021 Mar;23(3). doi: 10.3892/mmr.2021.11852. Epub 2021 Jan 26.
10
Research update on the association between SFRP5, an anti-inflammatory adipokine, with obesity, type 2 diabetes mellitus and coronary heart disease.SFRP5，一种抗炎脂肪因子，与肥胖、2 型糖尿病和冠心病关系的研究进展。
J Cell Mol Med. 2020 Mar;24(5):2730-2735. doi: 10.1111/jcmm.15023. Epub 2020 Jan 31.
本文引用的文献
1
Mechanisms of angiogenesis in a Curculigoside A-treated rat model of cerebral ischemia and reperfusion injury.仙茅苷A治疗的大鼠脑缺血再灌注损伤模型中的血管生成机制
Toxicol Appl Pharmacol. 2015 Nov 1;288(3):313-21. doi: 10.1016/j.taap.2015.08.003. Epub 2015 Aug 14.
2
Wnt5a attenuates hypoxia-induced pulmonary arteriolar remodeling and right ventricular hypertrophy in mice.Wnt5a可减轻小鼠低氧诱导的肺小动脉重塑和右心室肥厚。
Exp Biol Med (Maywood). 2015 Dec;240(12):1742-51. doi: 10.1177/1535370215584889. Epub 2015 May 7.
3
Wnt5a-induced Wnt1-inducible secreted protein-1 suppresses vascular smooth muscle cell apoptosis induced by oxidative stress.Wnt5a诱导的Wnt1诱导分泌蛋白-1抑制氧化应激诱导的血管平滑肌细胞凋亡。
Arterioscler Thromb Vasc Biol. 2014 Nov;34(11):2449-56. doi: 10.1161/ATVBAHA.114.303922. Epub 2014 Sep 11.
4
Wnt5a is associated with the differentiation of bone marrow mesenchymal stem cells in vascular calcification by connecting with different receptors.Wnt5a通过与不同受体结合，在血管钙化过程中与骨髓间充质干细胞的分化相关。
Mol Med Rep. 2014 Oct;10(4):1985-91. doi: 10.3892/mmr.2014.2449. Epub 2014 Aug 5.
5
Prevention of abdominal aortic aneurysm by anti-microRNA-712 or anti-microRNA-205 in angiotensin II-infused mice.血管紧张素 II 输注小鼠中抗 microRNA-712 或抗 microRNA-205 预防腹主动脉瘤。
Arterioscler Thromb Vasc Biol. 2014 Jul;34(7):1412-21. doi: 10.1161/ATVBAHA.113.303134. Epub 2014 May 8.
6
microRNA profiling in patients with abdominal aortic aneurysms: the significance of miR-155.腹主动脉瘤患者的 microRNA 谱分析：miR-155 的意义。
Clin Sci (Lond). 2014 Jun;126(11):795-803. doi: 10.1042/CS20130599.
7
Pathogenesis of abdominal aortic aneurysms: microRNAs, proteases, genetic associations.腹主动脉瘤的发病机制：微小 RNA、蛋白酶、遗传关联。
Annu Rev Med. 2014;65:49-62. doi: 10.1146/annurev-med-101712-174206. Epub 2013 Nov 21.
8
MicroRNA-29b regulation of abdominal aortic aneurysm development.微小 RNA-29b 调控腹主动脉瘤的发展。
Trends Cardiovasc Med. 2014 Jan;24(1):1-6. doi: 10.1016/j.tcm.2013.05.002. Epub 2013 Jul 18.
9
Micromanaging abdominal aortic aneurysms.精细化管理腹主动脉瘤。
Int J Mol Sci. 2013 Jul 11;14(7):14374-94. doi: 10.3390/ijms140714374.
10
Identification and characterization of microRNAs in vascular smooth muscle cells from patients with abdominal aortic aneurysms.鉴定和分析腹主动脉瘤患者血管平滑肌细胞中的 microRNAs。
J Vasc Surg. 2014 Jan;59(1):202-9. doi: 10.1016/j.jvs.2013.02.244. Epub 2013 Jun 5.
Zotero 插件