Chen Danjun, Luo Cong
Department of Pharmacy, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
Department of Hematology, The First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, P.R. China.
Exp Ther Med. 2021 Nov;22(5):1249. doi: 10.3892/etm.2021.10684. Epub 2021 Sep 2.
Salidroside, an active ingredient of , exhibits antitumor effects in various types of cancer. However, the role of salidroside in chronic myeloid leukemia (CML) has not been elucidated. In the presents study, cell viability was assessed by CCK-8 assay, while apoptosis was detected by flow cytometry. Reverse transcription-quantitative PCR analysis was used to examine the expression levels of miR-140-5p in human CML cell lines. The expression levels of apoptosis and cell cycle-associated proteins and of the wnt5a/β-catenin signaling pathway were determined by western blot analysis. Bioinformatic analysis and luciferase reporter assays were employed to investigate the association between miR-140-5p and wnt5a. The results revealed that exposure of CML cells to salidroside (80 µM) inhibited cell proliferation and promoted apoptosis. In addition, salidroside treatment led to the upregulation of miR-140-5p expression. Furthermore, the inhibition of wnt5a/β-catenin signaling pathway and the pro-apoptotic effects induced by salidroside were attenuated by miR-140-5p silencing. Notably, wnt5a was revealed to be a direct target of miR-140-5p. The present findings indicated that salidroside exerted anti-CML effects through regulating miR-140-5p by suppressing the wnt5a/β-catenin signaling pathway. The present study provided evidence of the therapeutic role of salidroside in CML.
红景天苷是[某种物质]的一种活性成分，在多种癌症类型中均表现出抗肿瘤作用。然而，红景天苷在慢性髓性白血病（CML）中的作用尚未阐明。在本研究中，通过CCK - 8法评估细胞活力，同时采用流式细胞术检测细胞凋亡。运用逆转录定量PCR分析检测人CML细胞系中miR - 140 - 5p的表达水平。通过蛋白质印迹分析确定凋亡和细胞周期相关蛋白以及wnt5a/β - 连环蛋白信号通路的表达水平。采用生物信息学分析和荧光素酶报告基因检测来研究miR - 140 - 5p与wnt5a之间的关联。结果显示，将CML细胞暴露于红景天苷（80 µM）可抑制细胞增殖并促进细胞凋亡。此外，红景天苷处理导致miR - 140 - 5p表达上调。此外，miR - 140 - 5p沉默减弱了红景天苷诱导的wnt5a/β - 连环蛋白信号通路抑制及促凋亡作用。值得注意的是，wnt5a被证实为miR - 140 - 5p的直接靶点。本研究结果表明，红景天苷通过抑制wnt5a/β - 连环蛋白信号通路调控miR - 140 - 5p发挥抗CML作用。本研究为红景天苷在CML中的治疗作用提供了证据。
