Gai Wen-Tao, Yu Da-Peng, Wang Xin-Sheng, Wang Pei-Tao
Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China; Department of Urology, Yantai Municipal Laiyang Central Hospital, Laiyang, Shandong 265200, P.R. China.
Department of Urology, Jining No. 1 People's Hospital, Jining, Shandong 272000, P.R. China.
Oncol Lett. 2016 Oct;12(4):2880-2885. doi: 10.3892/ol.2016.5015. Epub 2016 Aug 16.
Ursolic acid is a type of pentacyclic triterpene compound with multiple pharmacological activities including cancer resistance, protection from liver injury, antisepsis, anti-inflammation and antiviral activity. The present study aimed to investigate the anticancer effect of ursolic acid. Ursolic acid activates cell apoptosis and its pro-apoptotic mechanism remains to be fully elucidated. Cell Counting kit-8 assays, flow cytometric analysis and analysis of caspase-3 and caspase-9 activity were used to estimate the anticancer effect of ursolic acid on DU145 prostate cancer cells. The protein expression of cytochrome , rho-associated protein kinase (ROCK), phosphatase and tensin homolog (PTEN) and cofilin-1 were examined using western blot analysis. In the present study, ursolic acid significantly suppressed cell growth and induced apoptosis, as well as increasing caspase-3 and caspase-9 activities of DU145 cells. Furthermore, cytoplasmic and mitochondrial cytochrome protein expression was significantly activated and suppressed, respectively, by ursolic acid. Ursolic acid significantly suppressed the ROCK/PTEN signaling pathway and inhibited cofilin-1 protein expression in DU145 cells. The results of the present study indicate that the anticancer effect of ursolic acid activates cell apoptosis through ROCK/PTEN mediated mitochondrial translocation of cofilin-1 in prostate cancer.
熊果酸是一种五环三萜类化合物，具有多种药理活性，包括抗癌、保护肝脏免受损伤、抗菌、抗炎和抗病毒活性。本研究旨在探讨熊果酸的抗癌作用。熊果酸可激活细胞凋亡，但其促凋亡机制仍有待充分阐明。采用细胞计数试剂盒-8法、流式细胞术分析以及半胱天冬酶-3和半胱天冬酶-9活性分析来评估熊果酸对DU145前列腺癌细胞的抗癌作用。使用蛋白质印迹分析检测细胞色素、Rho相关蛋白激酶（ROCK）、磷酸酶和张力蛋白同源物（PTEN）以及丝切蛋白-1的蛋白表达。在本研究中，熊果酸显著抑制细胞生长并诱导凋亡，同时增加DU145细胞的半胱天冬酶-3和半胱天冬酶-9活性。此外，熊果酸分别显著激活和抑制细胞质和线粒体细胞色素蛋白表达。熊果酸显著抑制ROCK/PTEN信号通路并抑制DU145细胞中丝切蛋白-1的蛋白表达。本研究结果表明，熊果酸的抗癌作用通过ROCK/PTEN介导的丝切蛋白-1在前列腺癌中的线粒体转位激活细胞凋亡。
