宫颈癌中组蛋白H3的低蛋白水解剪切
Low Proteolytic Clipping of Histone H3 in Cervical Cancer.
作者信息
Sandoval-Basilio Jorge, Serafín-Higuera Nicolás, Reyes-Hernandez Octavio D, Serafín-Higuera Idanya, Leija-Montoya Gabriela, Blanco-Morales Magali, Sierra-Martínez Monica, Ramos-Mondragon Roberto, García Silvia, López-Hernández Luz Berenice, Yocupicio-Monroy Martha, Alcaraz-Estrada Sofia L
机构信息
Área de investigación clínica, Unidad de Innovación Clínica y Epidemiológica de la Secretaría de Salud del estado de Guerrero, Acapulco, Gro., México.; Laboratorio de Biología Molecular, Universidad Hipócrates, Acapulco, Gro., México.
Laboratorio de Biología Celular, Unidad Ciencias de la Salud, Facultad de Odontología, Universidad Autónoma de Baja California Mexicali, BC, México.
出版信息
J Cancer. 2016 Aug 17;7(13):1856-1860. doi: 10.7150/jca.15605. eCollection 2016.
Chromatin in cervical cancer (CC) undergoes chemical and structural changes that alter the expression pattern of genes. Recently, a potential mechanism, which regulates gene expression at transcriptional levels is the proteolytic clipping of histone H3. However, until now this process in CC has not been reported. Using HeLa cells as a model of CC and human samples from patients with CC, we identify that the H3 cleavage was lower in CC compared with control tissue. Additionally, the histone H3 clipping was performed by serine and aspartyl proteases in HeLa cells. These results suggest that histone H3 clipping operates as part of post-translational modification system in CC.
宫颈癌（CC）中的染色质会发生化学和结构变化，从而改变基因的表达模式。最近，一种在转录水平调节基因表达的潜在机制是组蛋白H3的蛋白水解剪切。然而，迄今为止，CC中的这一过程尚未见报道。我们以HeLa细胞作为CC模型，并使用CC患者的人体样本，发现与对照组织相比，CC中H3的剪切作用较弱。此外，在HeLa细胞中，组蛋白H3的剪切是由丝氨酸蛋白酶和天冬氨酸蛋白酶完成的。这些结果表明，组蛋白H3剪切是CC中翻译后修饰系统的一部分。
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