Entwisle Blake, McMullan Simon, Bokiniec Phillip, Gross Simon, Chung Roger, Withford Michael
Department of Physics and Astronomy, Faculty of Science and Engineering, Macquarie University, Australia Centre for Ultrahigh bandwidth Devices for Optical Systems, Australia.
Australian School of Advanced Medicine, Faculty of Human Sciences, Macquarie University, Australia.
Biomed Opt Express. 2016 Aug 3;7(9):3211-3219. doi: 10.1364/BOE.7.003211. eCollection 2016 Sep 1.
Neuronal responses to infrared neural stimulation (INS) are explored at the single cell level using patch-clamp electrophysiology. We examined membrane and synaptic responses of solitary tract neurons recorded in acute slices prepared from the Sprague-Dawley rat. Neurons were stimulated using a compact 1890 nm waveguide laser with light delivered to a small target area, comparable to the size of a single cell, via a single-mode fiber. We show that infrared radiation increased spontaneous synaptic event frequency, and evoked steady-state currents and neuronal depolarization. The magnitude of the responses was proportional to laser output.
使用膜片钳电生理学在单细胞水平上探索神经元对红外神经刺激（INS）的反应。我们检查了从Sprague-Dawley大鼠制备的急性脑片中记录的孤束核神经元的膜反应和突触反应。使用紧凑型1890 nm波导激光器刺激神经元，通过单模光纤将光传递到与单个细胞大小相当的小目标区域。我们发现红外辐射增加了自发突触事件的频率，并诱发了稳态电流和神经元去极化。反应的幅度与激光输出成正比。
