Lin Chih-Ju, Li Feng-Chieh, Lee Yu-Yang, Tseng Te-Yu, Chen Wei-Liang, Hovhannisyan Vladimir, Kang Ning, Horton Nicholas G, Chiang Shu-Jen, Xu Chris, Lee Hsuan-Shu, Dong Chen-Yuan
Department of Physics, National Taiwan University, Taipei 106, Taiwan.
School of Applied and Engineering Physics, Cornell University, Ithaca, NY, 14853, USA.
Biomed Opt Express. 2016 Aug 19;7(9):3574-3584. doi: 10.1364/BOE.7.003574. eCollection 2016 Sep 1.
Hepatobiliary metabolism is one of the major functions of the liver. However, little is known of the relationship between the physiological location of the hepatocytes and their metabolic potential. By the combination of time-lapse multiphoton microscopy and first order kinetic constant image analysis, the hepatocellular metabolic rate of the model compound 6-carboxyfluorescein diacetate (6-CFDA) is quantified at the single cell level. We found that the mouse liver can be divided into three zones, each with distinct metabolic rate constants. The sinusoidal uptake coefficients k of Zones 1, 2, and 3 are respectively 0.239 ± 0.077, 0.295 ± 0.087, and 0.338 ± 0.133 min, the apical excreting coefficients k of Zones 1, 2, and 3 are 0.0117 ± 0.0052, 0.0175 ± 0.0052, and 0.0332 ± 0.0195 min, respectively. Our results show not only the existence of heterogeneities in hepatobiliary metabolism, but they also show that Zone 3 is the main area of metabolism.
肝胆代谢是肝脏的主要功能之一。然而，对于肝细胞的生理位置与其代谢潜能之间的关系，人们了解甚少。通过延时多光子显微镜和一级动力学常数图像分析相结合的方法，在单细胞水平上对模型化合物6-羧基荧光素二乙酸酯（6-CFDA）的肝细胞代谢率进行了量化。我们发现，小鼠肝脏可分为三个区域，每个区域具有不同的代谢速率常数。1区、2区和3区的窦状隙摄取系数k分别为0.239±0.077、0.295±0.087和0.338±0.133分钟，1区、2区和3区的顶端排泄系数k分别为0.0117±0.0052、0.0175±0.0052和0.0332±0.0195分钟。我们的研究结果不仅表明了肝胆代谢中存在异质性，还表明3区是主要的代谢区域。
