脂肪来源的间充质干细胞减少诱导性自身免疫性泪腺炎兔模型中的淋巴细胞浸润。

Adipose-Derived Mesenchymal Stem Cells Reduce Lymphocytic Infiltration in a Rabbit Model of Induced Autoimmune Dacryoadenitis.

作者信息

Li Xue, Lu Xiaoxiao, Sun Deming, Wang Xilian, Yang Liyuan, Zhao Shaozhen, Nian Hong, Wei Ruihua

机构信息

Tianjin Medical University Eye Hospital, Eye Institute & School of Optometry and Ophthalmology, Tianjin, China.

Doheny Eye Institute, and Department of Ophthalmology, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, California, United States.

出版信息

Invest Ophthalmol Vis Sci. 2016 Oct 1;57(13):5161-5170. doi: 10.1167/iovs.15-17824.

PURPOSE

To investigate the immunoregulatory roles of adipose-derived mesenchymal stem cells (ADSCs) in autoimmune dacryoadenitis.

METHODS

Rabbits were treated with ADSCs or phosphate-buffered solution on days 1, 3, 5, 7, and 9 after injection of activated peripheral blood lymphocytes, and clinical scores were determined by assessing tear production, break-up time, and fluorescein and hematoxylin and eosin staining. Inflammatory response was determined by measuring the expression of different mediators of inflammation in the lacrimal glands. The Th1/Th17-mediated autoreactive responses were evaluated by determining the proliferative response and the expression of cytokine genes and the lineage-determining transcription factors. The frequency of regulatory T cells (Tregs) was also examined.

RESULTS

The ADSC-treated rabbits showed decreased autoimmune responses, and the secretory function of their lacrimal gland was restored significantly. Treatment with ADSCs downregulated the Th1 and Th17 responses but enhanced Tregs function. In addition, ADSC treatment noticeably suppressed the expression of matrix metalloproteinase (MMP)-9, MPP-2, IL-1β, and IL-6, whereas it enhanced the expression of the anti-inflammatory cytokine IL-10.

CONCLUSIONS

Our results demonstrated that ADSC administration efficiently ameliorates autoimmune dacryoadenitis mainly via modulating Th1/Th17 responses.

目的

研究脂肪来源间充质干细胞（ADSCs）在自身免疫性泪腺炎中的免疫调节作用。

方法

在注射活化外周血淋巴细胞后的第1、3、5、7和9天，用ADSCs或磷酸盐缓冲溶液处理兔子，并通过评估泪液分泌、泪膜破裂时间以及荧光素和苏木精-伊红染色来确定临床评分。通过测量泪腺中不同炎症介质的表达来确定炎症反应。通过测定增殖反应、细胞因子基因表达和谱系决定转录因子来评估Th1/Th17介导的自身反应性。还检测了调节性T细胞（Tregs）的频率。