Koga Yasuhiko, Satoh Takahiro, Kaira Kyoichi, Koka Masashi, Hisada Takeshi, Hirato Junko, Altan Bolag, Yatomi Masakiyo, Ono Akihiro, Kamide Yosuke, Shimizu Yasuo, Aoki-Saito Haruka, Tsurumaki Hiroaki, Shimizu Kimihiro, Mogi Akira, Ishizuka Tamotsu, Yamada Masanobu, Dobashi Kunio
Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine, 3-39-15 sho-wa machi, Maebashi, Gunma, 371-8511, Japan.
Takasaki Advanced Radiation Research Institute, Japan Atomic Energy Agency, 1233 Watanuki-machi, Takasaki, Gunma, 370-1292, Japan.
Environ Health Prev Med. 2016 Nov;21(6):492-500. doi: 10.1007/s12199-016-0576-5. Epub 2016 Oct 3.
Studies have shown that inhaled mine dust, such as asbestos, can be translocated to various organs including the lymph nodes. Recently, we have established a protocol that enables us to identify inhaled elements using paraffin embedded lung specimens by in-air microparticle-induced X-ray emission (micro-PIXE). However, little research has examined the concentration of these inhaled fibers in various organs or the mechanisms of their translocation. In this study, we compared the concentration of inhaled fibers in the lung parenchyma to the concentration in the hilar lymph node as well as to determine the elemental spatial distribution of the inhaled fibers in a patient with occupational asbestos exposure.
Lung tissues and hilar lymph node in a patient with asbestos exposure were used in this study. Elemental analysis was performed by in-air micro-PIXE. Immunohistochemical analysis was performed using anti CD163, smooth muscle actin, vimentin and β-catenin antibody.
The analysis revealed that the amount of inhaled silicon was approximately 6 times higher in the lymph node than in the lungs. The spatial analysis showed that silicon, iron and aluminium were co-localized in the hilar lymph node. The immunohistochemical analysis showed localized agreement of the inhaled fibers with macrophages, smooth muscle actin, and vimentin in the hilar lymph node.
This study showed that in-air micro-PIXE could be useful for analyzing the elemental distribution and quantification of inhaled fibers in the human body. Furthermore, immunohistochemistry in combination with in-air micro-PIXE analyses may help to determine the mechanism of mine dust distribution in vivo.
研究表明，吸入的矿尘，如石棉，可转移至包括淋巴结在内的各种器官。最近，我们建立了一种方案，通过空气中微粒诱导X射线发射（微PIXE），利用石蜡包埋的肺标本识别吸入元素。然而，很少有研究检测这些吸入纤维在各种器官中的浓度或其转移机制。在本研究中，我们比较了职业性石棉暴露患者肺实质中吸入纤维的浓度与肺门淋巴结中的浓度，并确定了吸入纤维的元素空间分布。
本研究使用了一名石棉暴露患者的肺组织和肺门淋巴结。通过空气中微PIXE进行元素分析。使用抗CD163、平滑肌肌动蛋白、波形蛋白和β-连环蛋白抗体进行免疫组织化学分析。
分析显示，淋巴结中吸入硅的量约为肺中的6倍。空间分析表明，硅、铁和铝在肺门淋巴结中共定位。免疫组织化学分析显示，肺门淋巴结中吸入纤维与巨噬细胞、平滑肌肌动蛋白和波形蛋白局部一致。
本研究表明，空气中微PIXE可用于分析人体中吸入纤维的元素分布和定量。此外，免疫组织化学与空气中微PIXE分析相结合可能有助于确定矿尘在体内的分布机制。
