Kawashima H, Sasaki K, Nakagawa M, Mori T, Makino S, Matumoto M
Kitasato Arch Exp Med. 1989 Apr;62(1):39-44.
Japanese sera were shown by enzyme-linked immunosorbent assay (ELISA) to contain frequently antibodies against allantoic fluid, confirming the results obtained in our previous study on ELISA for the detection of mumps antibodies. Chromatographic analysis with Sephacryl S-300 of the allantoic fluid antigen used for ELISA suggested the antigens for these antibodies in Japanese sera to be macromolecular proteins. Comparative absorption tests with allantoic fluid and ovomacroglobulin were therefore carried out on serum samples positive in ELISA with allantoic fluid antigen, and ovomacroglobulin was identified to be the antigenic component for antibodies to allantoic fluid antigen in Japanese sera.
通过酶联免疫吸附测定（ELISA）表明，日本血清中经常含有抗尿囊液抗体，这证实了我们先前关于ELISA检测腮腺炎抗体的研究结果。用Sephacryl S-300对用于ELISA的尿囊液抗原进行色谱分析，结果表明日本血清中这些抗体的抗原是大分子蛋白质。因此，对用尿囊液抗原进行ELISA检测呈阳性的血清样本，用尿囊液和卵巨球蛋白进行了比较吸收试验，结果确定卵巨球蛋白是日本血清中抗尿囊液抗原抗体的抗原成分。
