Christensen Mette, Madsen Rikke Fogt, Møller Line Rosengreen, Knudsen Cindy Soendersoe, Samson Mie Hessellund
a Faculty of Health Science , VIA University College , Aarhus , Denmark.
b Department of Clinical Biochemistry , Aarhus University Hospital , Aarhus , Denmark.
Scand J Clin Lab Invest. 2016 Dec;76(8):653-656. doi: 10.1080/00365513.2016.1230887. Epub 2016 Oct 5.
The aim of this study was to investigate and compare the stability of adrenocorticotrophic hormone (ACTH) in whole blood stored on ice and at room temperature for up to 48 hours. This study differs from previous studies by a larger data material.
EDTA-blood samples from 30 patients were collected, aliquoted and stored on ice or at room temperature for 0, 2, 4, 24, or 48 h before centrifugation, and the plasma was stored frozen until analysis. All samples were analyzed using an automated electrochemiluminescence immunoassay on cobas 6000 e601. The change in ACTH concentration was illustrated as ACTH recovery compared to standard conditions defined as samples stored immediately on ice, centrifuged and plasma frozen within 1 h. A change in ACTH concentration of more than 10% was considered to be of clinical relevance.
The results showed no clinically relevant change in ACTH recovery for up to 4 h compared to standard conditions. For samples stored at room temperature for 4 h, a significant (p < .0001) relative mean change in ACTH concentrations of -4.3% was observed.
The comparison between samples stored at room temperature for up to 4 h and standard conditions showed that ACTH samples do not require cooling until centrifugation, if a mean difference in ACTH concentration of -4.3%, between the individual results, can be accepted.
本研究旨在调查和比较促肾上腺皮质激素（ACTH）在冰上和室温下储存长达48小时的全血中的稳定性。本研究与以往研究的不同之处在于数据材料更多。
采集30例患者的乙二胺四乙酸（EDTA）抗凝血样本，进行分装，在离心前于冰上或室温下储存0、2、4、24或48小时，血浆冷冻保存直至分析。所有样本均使用cobas 6000 e601全自动电化学发光免疫分析法进行分析。将促肾上腺皮质激素浓度的变化表示为与标准条件相比的促肾上腺皮质激素回收率，标准条件定义为样本立即在冰上储存、1小时内离心并冷冻血浆。促肾上腺皮质激素浓度变化超过10%被认为具有临床相关性。
结果显示，与标准条件相比，长达4小时内促肾上腺皮质激素回收率无临床相关变化。对于在室温下储存4小时的样本，观察到促肾上腺皮质激素浓度相对平均变化显著（p <.0001），为-4.3%。
室温下储存长达4小时的样本与标准条件之间的比较表明，如果个体结果之间促肾上腺皮质激素浓度的平均差异为-4.3%可以接受，那么促肾上腺皮质激素样本在离心前不需要冷却。
