Toprak Burak, Yalcin Hulya, Arı Elif, Colak Ayfer
1 Department of Clinical Biochemistry, Silopi State hospital, Silopi, Şırnak, Turkey.
2 Department of Clinical Biochemistry, Tepecik Teaching and Research Hospital, Izmir, Turkey.
Ann Clin Biochem. 2016 Nov;53(6):699-701. doi: 10.1177/0004563216636898. Epub 2016 Sep 28.
Background As plasma is the recommended sample type for Roche adrenocorticotropic hormone (ACTH) assay, we evaluated the effect of EDTA concentration on Cobas ACTH assay. Methods Samples containing twofold and fourfold higher concentrations of EDTA were prepared by adding plasma to empty K2EDTA tubes and by making under-filled EDTA tubes. All measurements were performed with four replicates. Results Increased EDTA concentration resulted in a significant decrease in ACTH concentration. Fifty-per cent-filled EDTA tube showed 19% decrease in ACTH concentration and 25% filled EDTA tube showed 50% decrease in ACTH concentration. Conclusion We recommend that inadequately filled EDTA specimens should be rejected when using Cobas ACTH assay.
背景 由于血浆是罗氏促肾上腺皮质激素（ACTH）检测推荐的样本类型，我们评估了乙二胺四乙酸（EDTA）浓度对 Cobas ACTH 检测的影响。方法 通过将血浆加入空的 K2EDTA 管以及制作未装满 EDTA 的管来制备含有两倍和四倍高浓度 EDTA 的样本。所有测量均重复进行四次。结果 EDTA 浓度增加导致 ACTH 浓度显著降低。50%装满的 EDTA 管中 ACTH 浓度降低了 19%，25%装满的 EDTA 管中 ACTH 浓度降低了 50%。结论 我们建议在使用 Cobas ACTH 检测时，应拒收未装满的 EDTA 样本。
