Finbarrs-Bello Elizabeth, Obikili Emmanuel Nebeuwa, Anayochukwu Esom Emmanuel, Godson Anyanwu Emeka
Department of Anatomy, College of Medicine, University of Nigeria, UNEC, Enugu, Nigeria; Department of Anatomy, Ebonyi State University, Abakaliki Ebonyi State, Nigeria.
Department of Anatomy, College of Medicine, University of Nigeria, UNEC, Enugu, Nigeria.
Open Access Maced J Med Sci. 2016 Sep 15;4(3):337-341. doi: 10.3889/oamjms.2016.061. Epub 2016 Aug 6.
This study evaluated the curative potential of in the treatment of schizophrenia using an NMDA-receptor antagonist-induced schizophrenic Wistar rat model.
Twenty-five adult Wistar rats of both sexes of average weights 180 g were divided into two groups: control and schizophrenic rat models. The controls received 0.1 ml of 0. 9% saline, while schizophrenia was induced in models using 25 mg/kg of ketamine hydrochloride (i.p.) for 7 days. On the 8 day models were divided into group's k1, k2, k3 and k4 of 5 rats each. K1 and the controls were sacrificed then, groups k2 and k3 were treated with 5 mg/kg and 10 mg/kg aqueous leaf extract of while, k4 (standard) received 25 mg/kg of chlorpromazine orally for 28 days. Amygdala were harvested, processed and stained with Haematoxylin and Eosin (H &E) stain, Neuron-specific enolase (NSE) marker was also used to monitor the curative effect on the amygdala.
Degenerative changes and increased NSE immunoreactivity were observed in the untreated models. Extract-treated models showed normal amygdala and negative NSE immunoreactivity while chlorpromazine treated models revealed decreased NSE immunoreactivity.
Crinum giganteum extracts exhibits better curative effect than the standard antipsychotic agent.
本研究使用N-甲基-D-天冬氨酸（NMDA）受体拮抗剂诱导的精神分裂症Wistar大鼠模型，评估[植物名称未给出]在治疗精神分裂症方面的治疗潜力。
将25只平均体重180克的成年雌雄Wistar大鼠分为两组：对照组和精神分裂症大鼠模型组。对照组接受0.1毫升0.9%的生理盐水，而模型组使用25毫克/千克盐酸氯胺酮腹腔注射7天来诱导精神分裂症。在第8天，将模型组分为k1、k2、k3和k4组，每组5只大鼠。然后处死k1组和对照组，k2组和k3组分别用5毫克/千克和10毫克/千克的[植物名称未给出]水叶提取物治疗，而k4组（标准组）口服25毫克/千克氯丙嗪，持续28天。采集杏仁核，进行处理并用苏木精和伊红（H&E）染色，还使用神经元特异性烯醇化酶（NSE）标记物来监测对杏仁核的治疗效果。
在未治疗的模型中观察到退行性变化和NSE免疫反应性增加。提取物治疗的模型显示杏仁核正常且NSE免疫反应性为阴性，而氯丙嗪治疗的模型显示NSE免疫反应性降低。
巨型文殊兰提取物表现出比标准抗精神病药物更好的治疗效果。
