Li X Z, Han Y, Tian J, Ren X, Ma X, Ma M
Department of Pathogen Biology, Jining Medical College, Jining, Shandong, China.
Department of Hematology and Oncology, Institute of Basic Medicine, Shandong, Academy of Medical Science, Ji'nan, Shandong, China.
Genet Mol Res. 2016 Aug 30;15(3):gmr9001. doi: 10.4238/gmr.15039001.
To determine the cytotoxic effect of lymphocytes activated by melanoma-associated antigen 3 (MAGE-3)-sensitized dendritic cells (DCs) on BIU-87 tumor cells, and to evaluate the possibility of MAGE-3-peptide-pulsed DCs as a vaccine in bladder cancer immunotherapy, the proliferation of T cells and the activity of cytotoxic T lymphocytes (CTLs) were examined by the MTT method. CTLs were induced by MAGE-3-sensitized DCs, or by ovalbumin (OVA) peptide and non-sensitized DCs as controls, respectively. The results indicated that MAGE-3-sensitized DCs have the ability to promote the proliferation of T cells as well as the cytotoxic activity of CTLs on bladder cancer cells in comparison with OVA peptide and non-sensitized DCs. In other words, DCs sensitized by the MAGE-3 antigen peptide could obviously upregulate the proliferation of T cells, which resulted in the growth inhibition of bladder cancer BIU-87 cells. In addition, MAGE-3-sensitized DCs played an important role in inhibiting the growth of human BIU-87 tumor xenografts in nude mice.
为了确定黑色素瘤相关抗原3（MAGE-3）致敏的树突状细胞（DCs）激活的淋巴细胞对BIU-87肿瘤细胞的细胞毒性作用，并评估MAGE-3肽脉冲DCs作为膀胱癌免疫治疗疫苗的可能性，采用MTT法检测T细胞的增殖和细胞毒性T淋巴细胞（CTLs）的活性。CTLs分别由MAGE-3致敏的DCs或卵清蛋白（OVA）肽和未致敏的DCs作为对照诱导产生。结果表明，与OVA肽和未致敏的DCs相比，MAGE-3致敏的DCs具有促进T细胞增殖以及CTLs对膀胱癌细胞的细胞毒性活性的能力。换句话说，MAGE-3抗原肽致敏的DCs可明显上调T细胞的增殖，从而导致膀胱癌BIU-87细胞的生长抑制。此外，MAGE-3致敏的DCs在抑制裸鼠体内人BIU-87肿瘤异种移植瘤的生长中发挥了重要作用。
