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[来自小鼠联会复合体分离组分的DNA的各种特性]

[Various properties of DNA from isolated fractions of the mouse synaptonemal complexes].

作者信息

Karpova O I, Safronov V V, Zaĭtseva S P, Bogdanov Iu F

出版信息

Mol Biol (Mosk). 1989 Mar-Apr;23(2):571-9.

PMID:2770732
Abstract

A fraction of synaptonemal complexes (SC) isolated from mouse spermatocytes has been electrophoretically purified in agarose gel. The DNA from the SC fraction constitutes approximately 0.5% of total nuclear DNA, and its molecules have length heterogeneity from 1 k.b. to 20 k.b. The content of beta-globin gene is the same in DNA from the SC fraction and in total nuclear DNA. The specificity of DNA from the SC fraction is manifested by higher contents of the repeated alternative sequences GT/CA and B1-sequence that is probably due to the processes of genetic meiotic recombination.

摘要

从小鼠精母细胞中分离出的一部分联会复合体(SC)已在琼脂糖凝胶中进行了电泳纯化。SC组分中的DNA约占核DNA总量的0.5%,其分子长度具有异质性,从1千碱基对到20千碱基对不等。SC组分DNA中的β-珠蛋白基因含量与核DNA总量中的相同。SC组分DNA的特异性表现为重复的交替序列GT/CA和B1序列的含量较高,这可能是由于减数分裂遗传重组过程所致。

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