Dadashev S Ia, Bogdanov Iu F, Gorach G G, Kolomiets O L, Karpova O I
Tsitologiia. 1993;35(6-7):109-12.
A method of isolation of synaptonemal complexes (SC) from mouse, rat and Syrian hamster spermatocytes is described. A fraction of pachytene spermatocyte nuclei was obtained by centrifugation of the testis homogenate in stepwise sucrose gradient and then lysed. The resulting chromatine was hydrolysed with DNAse II, and a fraction of isolated SCs was obtained by ultracentrifugation of the hydrolysate. The method can be applied for obtaining the SC fraction from spermatocytes sufficient for cytological, biochemical and molecular biology studies.
本文描述了一种从小鼠、大鼠和叙利亚仓鼠精母细胞中分离联会复合体（SC）的方法。通过在逐步蔗糖梯度中对睾丸匀浆进行离心，获得粗线期精母细胞核的一部分，然后将其裂解。所得染色质用DNA酶II水解，通过对水解产物进行超速离心获得一部分分离的SC。该方法可用于从精母细胞中获得足以进行细胞学、生物化学和分子生物学研究的SC组分。
