Rakhimova G M, Troitskiĭ A V, Klikunova I N, Antonov A S
Mol Biol (Mosk). 1989 May-Jun;23(3):830-42.
The variable 260 base long region from the interior of 18S rRNA of 14 plant species was determined by chain termination method with the use of reverse transcriptase. The hairpin revealed in this region appeared to be conservative in all species compared. Thermodynamic stability of such hairpin is lower than of an alternative structure with different base pairing mode. From sequence data dendrograms were produced by clustering algorithms and by the compatibility method. In addition to the plant sequences these dendrograms included also the homologous regions from yeast and Xenopus 18S rRNAs. The compatibility method seems to be more reliable. Inferences were drawn on relations between gymnosperms and angiosperms, monocots and dicots on the bases of the analysis of this tree.
采用链终止法并利用逆转录酶，测定了14种植物18S核糖体RNA内部260个碱基长的可变区域。在所比较的所有物种中，该区域出现的发夹结构似乎是保守的。这种发夹结构的热力学稳定性低于具有不同碱基配对模式的另一种结构。根据序列数据，通过聚类算法和兼容性方法生成了系统发育树状图。除了植物序列外，这些树状图还包括酵母和非洲爪蟾18S核糖体RNA的同源区域。兼容性方法似乎更可靠。基于对这棵树的分析，得出了裸子植物与被子植物、单子叶植物与双子叶植物之间关系的推论。
