James S A, Collins M D, Roberts I N
Genetics and Microbiology Department, Norwich Laboratory, Colney, U.K.
Yeast. 1994 Jul;10(7):871-81. doi: 10.1002/yea.320100703.
The phylogenetic interrelationship of species of the genus Zygosaccharomyces was examined by 18S rRNA gene sequencing. Comparative analysis of the sequence data revealed the genus to consist of a number of distinct subdivisions. The most prevalent species associated with food spoilage, Z. bailii, Z. bisporus and Z. rouxii, along with Z. mellis were found to form one subdivision. Zygosaccharomyces cidri and Z. fermentati formed a distinct species pair, as did Z. microellipsoides and Z. mrakii. Zygosaccharomyces florentinus formed a separate line displaying no specific relationship with any of the other Zygosaccharomyces species examined. Comparison with nine published ascosporogenous yeast 18S rRNA gene sequences showed that Z. microellipsoides and Z. mrakii were genealogically very close to Torulaspora delbrueckii (both displaying 99.8% 18S rRNA sequence similarity), raising the possibility that these two Zygosaccharomyces species should be moved to the genus Torulaspora. The topologies of trees derived from complete 18S rRNA gene sequences and from individual domains within the gene were compared and the implications of using partial sequence data for inferring phylogenetic relationships discussed.
通过18S rRNA基因测序研究了接合酵母属物种间的系统发育关系。对序列数据的比较分析表明，该属由许多不同的亚群组成。发现与食品腐败相关的最常见物种巴氏接合酵母、双孢接合酵母、鲁氏接合酵母以及蜂蜜接合酵母形成一个亚群。柠檬接合酵母和发酵接合酵母形成一个独特的物种对，微小椭圆接合酵母和马克接合酵母也是如此。弗洛伦廷接合酵母形成一条单独的谱系，与所研究的任何其他接合酵母属物种均无特定关系。与九个已发表的产子囊酵母18S rRNA基因序列进行比较，结果显示微小椭圆接合酵母和马克接合酵母在谱系上与德氏有孢圆酵母非常接近（两者的18S rRNA序列相似性均为99.8%），这增加了将这两个接合酵母属物种转移到有孢圆酵母属的可能性。比较了由完整18S rRNA基因序列和该基因内各个结构域推导的树的拓扑结构，并讨论了使用部分序列数据推断系统发育关系的意义。
